ISOLATION OF CDNA CLONES FOR GENES THAT ARE EXPRESSED DURING LEAF SENESCENCE IN BRASSICA-NAPUS - IDENTIFICATION OF A GENE ENCODING A SENESCENCE-SPECIFIC METALLOTHIONEIN-LIKE PROTEIN
ISOLATION OF CDNA CLONES FOR GENES THAT ARE EXPRESSED DURING LEAF SENESCENCE IN BRASSICA-NAPUS - IDENTIFICATION OF A GENE ENCODING A SENESCENCE-SPECIFIC METALLOTHIONEIN-LIKE PROTEIN
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DOI：
10.1104/pp.105.3.839
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发表时间：
1994-07-01
期刊：
影响因子：
7.4
通讯作者：
BUCHANANWOLLASTON, V
中科院分区：
文献类型：
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作者：
BUCHANANWOLLASTON, V
通过对从不同衰老阶段的叶子中分离的RNA制成的cDNA文库进行差异筛选，鉴定了代表甘蓝型油菜叶子衰老过程中表达的基因的cDNA克隆。通过Northern印迹分析检查这些基因在叶子发育的不同阶段的表达，并观察到几种不同的表达模式。其中一个克隆 LSC54 代表了一种在叶子衰老过程中高水平表达的基因。对该基因的分析表明在花和衰老的叶子中都有很强的表达。 LSC54 cDNA 的 DNA 序列分析表明推导的氨基酸序列与先前在植物中鉴定的几种金属硫蛋白样蛋白之间存在相似性。
cDNA clones representing genes that are expressed during leaf senescence in Brassica napus were identified by differential screening of a cDNA library made from RNA isolated from leaves at different stages of senescence. The expression of these genes at different stages of leaf development was examined by northern blot analysis, and several different patterns of expression were observed. One of the clones, LSC54, represented a gene that is expressed at high levels during leaf senescence. Analysis of this gene indicated strong expression in flowers as well as in senescing leaves. DNA sequence analysis of the LSC54 cDNA indicated a similarity between the deduced amino acid sequence and several metallothionein-like proteins previously identified in plants.