THE MIGRATION OF LYMPHOCYTES ACROSS SPECIALIZED VASCULAR ENDOTHELIUM .4. PREDNISOLONE ACTS AT SEVERAL POINTS ON THE RE-CIRCULATION PATHWAYS OF LYMPHOCYTES
THE MIGRATION OF LYMPHOCYTES ACROSS SPECIALIZED VASCULAR ENDOTHELIUM .4. PREDNISOLONE ACTS AT SEVERAL POINTS ON THE RE-CIRCULATION PATHWAYS OF LYMPHOCYTES
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DOI：
10.1016/0008-8749(82)90190-3
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发表时间：
1982-01-01
影响因子：
4.3
通讯作者：
FORD, WL
中科院分区：
文献类型：
--
作者：
COX, JH;FORD, WL
将来自同源大鼠供体的放射性标记的胸导管淋巴细胞静脉注射到受体中，受体先前以1mg /h的速度连续静脉注射[免疫药物]强的松龙，持续15-18小时。用闪烁计数和放射自显影法定量比较强的松龙治疗组和对照组标记淋巴细胞的组织分布和再循环情况。强的松龙最突出的作用是阻碍淋巴细胞在血液正常分布的组织内的再循环，即骨髓、脾脏和淋巴结。尽管泼尼松龙几乎完全冻结了淋巴细胞交通，但循环淋巴细胞并未被杀死。强的松龙的第二个作用是削弱淋巴细胞从血液流入淋巴结。强的松龙抑制淋巴细胞进入的程度在不同淋巴结组有所不同；先前的抗原刺激完全使淋巴结免于这种抑制。泼尼松龙治疗的大鼠淋巴细胞通过高内皮小静脉壁的时间更长。强的松龙的第三个作用是增加淋巴细胞通过血窦内皮从血液进入骨髓的速率。淋巴细胞的运输对免疫反应的开始和发展至关重要。这里所描述的淋巴细胞运输的阻滞可能在很大程度上有助于强的松龙的免疫抑制作用。
Radioactively labeled thoracic duct lymphocytes from syngeneic rat donors were injected i.v. into recipients which had been given a continuous i.v. infusion of [the immunologic drug] prednisolone at 1 mg/h for 15-18 h previously. The tissue distribution and recirculation into lymph of the labeled lymphocytes were compared quantitatively in the prednisolone-treated and control recipients by scintillation counting and autoradiography. The most prominent effect of prednisolone was to retard recirculating lymphocytes within the tissues to which they are normally distributed by the blood, i.e., the bone marrow, the spleen and the lymph nodes. Although lymphocyte traffic was almost completely frozen by prednisolone, recirculating lymphocytes were not killed. A 2nd effect of prednisolone was to impair the influx of lymphocytes from the blood into lymph nodes. Different groups of lymph nodes varied in the extent to which prednisolone inhibited the entry of lymphocytes; previous antigenic stimulation completely exempted lymph nodes from this inhibition. Lymphocytes took a longer time to cross the walls of high endothelial venules in the lymph nodes of prednisolone-treated rats. A 3rd effect of prednisolone was to increase the rate at which lymphocytes entered the bone marrow from the blood by crossing sinusoidal endothelium. [The traffic of lymphocytes is vitually important to the initiation and development of the immune response. The arrest of lymphocyte traffic as described here may contribute substantially to the immunsuppressive effects of prednisolone.].