Mycoplasma hominis and Mycoplasma genitalium in the Vaginal Microbiota and Persistent High-Risk Human Papillomavirus Infection.
Mycoplasma hominis and Mycoplasma genitalium in the Vaginal Microbiota and Persistent High-Risk Human Papillomavirus Infection.
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DOI：
10.3389/fpubh.2017.00140
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发表时间：
2017
影响因子：
5.2
通讯作者：
ACCME Research Group
中科院分区：
文献类型：
--
作者：
Adebamowo SN;Ma B;Zella D;Famooto A;Ravel J;Adebamowo C;ACCME Research Group
最近的研究表明，阴道微环境在高危型人乳头瘤病毒（hrHPV）感染的持续存在中起作用，从而导致宫颈癌的发生。此外，已经表明，一些支原体是有效的甲基化剂，并可能通过hrHPV和宫颈体细胞的甲基化促进致癌。我们研究了支原体的流行和持久性之间的关系。在阴道微生物群中，以及普遍和持续的hrHPV感染。我们检查了194名使用SPF 25/LiPA 10检测hrHPV感染的尼日利亚妇女，并通过对16 S rRNA基因的V3-V4高变区进行测序，在其阴道微生物群中鉴定了生殖支原体和人型支原体。我们定义了M的患病率。genitalium、M.人型HPV和hrHPV基于基线测试的阳性结果，而持续性定义为来自两个连续测试的阳性结果。我们使用精确的逻辑回归模型来估计支原体属之间的关联。和hrHPV感染。研究参与者的平均（SD）年龄为38（8）岁，71%为HIV阳性，30%为M。生殖器阳性率为45%。人型HPV阳性，基线时40% hrHPV阳性。在随访中，16%的妇女对M仍呈阳性。生殖器M.人型HPV感染率为31%。持续性M.人型和持续性hrHPV（OR 8.78，95% CI 1.49-51.6，p 0.01）。HIV阳性和持续性M。与两者均为阴性的女性相比，人持续hrHPV感染的几率增加了3倍（OR 3.28，95% CI 1.31-8.74，p 0.008）。我们发现持续性M。在这项研究中，阴道微生物群中的人型和持续的hrHPV，但我们不能排除反向因果关系。我们的发现需要在更大的纵向研究中重复，如果得到证实，可能具有重要的诊断和治疗意义。
Recent studies have suggested that the vaginal microenvironment plays a role in persistence of high-risk human papillomavirus (hrHPV) infection and thus cervical carcinogenesis. Furthermore, it has been shown that some mycoplasmas are efficient methylators and may facilitate carcinogenesis through methylation of hrHPV and cervical somatic cells. We examined associations between prevalence and persistence of Mycoplasma spp. in the vaginal microbiota, and prevalent as well as persistent hrHPV infections. We examined 194 Nigerian women who were tested for hrHPV infection using SPF25/LiPA10 and we identified Mycoplasma genitalium and Mycoplasma hominis in their vaginal microbiota established by sequencing the V3–V4 hypervariable regions of the 16S rRNA gene. We defined the prevalence of M. genitalium, M. hominis, and hrHPV based on positive result of baseline tests, while persistence was defined as positive results from two consecutive tests. We used exact logistic regression models to estimate associations between Mycoplasma spp. and hrHPV infections. The mean (SD) age of the study participants was 38 (8) years, 71% were HIV positive, 30% M. genitalium positive, 45% M. hominis positive, and 40% hrHPV positive at baseline. At follow-up, 16% of the women remained positive for M. genitalium, 30% for M. hominis, and 31% for hrHPV. There was a significant association between persistent M. hominis and persistent hrHPV (OR 8.78, 95% CI 1.49–51.6, p 0.01). Women who were positive for HIV and had persistent M. hominis had threefold increase in the odds of having persistent hrHPV infection (OR 3.28, 95% CI 1.31–8.74, p 0.008), compared to women who were negative for both. We found significant association between persistent M. hominis in the vaginal microbiota and persistent hrHPV in this study, but we could not rule out reverse causation. Our findings need to be replicated in larger, longitudinal studies and if confirmed, could have important diagnostic and therapeutic implications.