Ambient pulsatile pressure modulates endothelial cell proliferation.
Ambient pulsatile pressure modulates endothelial cell proliferation.
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环境脉动压力调节内皮细胞增殖。
DOI：
10.1006/jmcc.1997.0625
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发表时间：
1998
影响因子：
5
通讯作者：
Sumpio,BE
中科院分区：
文献类型：
--
作者：
Vouyouka,AG;Powell,RJ;Ricotta,J;Chen,H;Dudrick,DJ;Sawmiller,CJ;Dudrick,SJ;Sumpio,BE
过去十年的许多研究表明，循环力如剪切应力和循环应变可以影响内皮细胞（EC）表型。然而，我们对压力对EC的体外效应知之甚少。为了研究这一点，在精心调节CO2/空气环境的定制压力室中培养牛主动脉EC。EC暴露在大气、静态（135 mmHg）或脉动压力（160/110 mmHg）下。由计算机控制的电磁阀与压力管线串联，保持脉冲压力频率为60 cycles/min。通过胰蛋白酶释放后第1、3、5天的细胞计数和3h -胸腺嘧啶掺入测定EC的增殖。到第5天，两个压力组的细胞数量都显著减少，胸腺嘧啶研究证实了这一点。LDH活性研究未观察到细胞形态和细胞活力的变化。为了研究这种影响的机理，将三种压力条件下的EC条件介质转移到未暴露的对照EC中。用脉冲压力条件下培养的EC的条件培养基处理的对照组显示出明显的细胞生长抑制。这一发现表明，暴露于脉动压力下的EC分泌一种具有生长抑制特性的自分泌因子。Northern blot分析和抗体中和研究表明，这种作用不是由生长因子tgf - β和IL-1介导的。
Many studies over the last decade have indicated that circulatory forces such as shear stress and cyclic strain can influence the endothelial cell (EC) phenotype. However, very little is known about thein vitroeffects of pressure on EC. To study this, cultured bovine aortic EC were grown in custom designed pressure chambers with carefully regulated CO2/air environment. EC were exposed to either atmospheric, static (135 mmHg) or pulsatile pressure (160/110 mmHg). A pulsed pressure frequency of 60 cycles/min was maintained by computer-controlled solenoid valves, placed in series with pressure lines. EC proliferation was determined both by cell count after trypsin release on days 1,3 and 5 and by3H-thymidine incorporation. By day 5, a significant decrease in cell number occurred in both pressure groups, confirmed by the thymidine studies. No changes were observed in cell morphology and cell viability as assessed by LDH activity studies. To investigate the mechanism of this effect, EC conditioned media from the three pressure conditions were transferred to non-exposed, control EC. Significant cell growth inhibition was demonstrated in the control EC group treated with conditioned media from EC cultured under pulsatile pressure conditions. This finding suggests that EC exposed to pulsatile pressure secrete an autocrine factor with growth inhibitory properties. This effect was not mediated by the growth factors TGFβand IL-1 as shown by Northern blot analysis and antibody-neutralization studies.