COMPLETE DEVELOPMENT OF HEPATIC STAGES OF PLASMODIUM-FALCIPARUM INVITRO
COMPLETE DEVELOPMENT OF HEPATIC STAGES OF PLASMODIUM-FALCIPARUM INVITRO
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DOI：
10.1126/science.3880923
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
LANGLOIS, P
中科院分区：
文献类型：
--
作者：
MAZIER, D;BEAUDOIN, RL;LANGLOIS, P
开发了一种体外模型来研究恶性疟原虫的肝期，恶性疟原虫是唯一对人类致命的疟疾寄生虫。用巴西和非洲恶性疟原虫株的子孢子接种人肝细胞的原代培养物。接种后第 1 天到第 7 天，荧光标记和吉姆萨染色制剂的检查表明存在许多细胞内寄生虫。在 3 组独立的实验中，发现所有培养物都感染了多达 650 个肝脏裂殖体，尺寸高达 40 μm。添加红细胞后，第12天和第13天通过免疫荧光测定检测到红细胞内形式的恶性疟原虫，表明肝循环已完成。
An in vitro model was developed to study the hepatic phase of P. falciparum, the only malaria parasite lethal to man. Primary cultures of human hepatocytes were inoculated with sporozoites of Brazilian and African strains of P. falciparum. On days 1 through 7 after inoculation examination of fluorescence-labeled and Giemsa-stained preparations demonstrated the presence of many intracellular parasites. In 3 separate sets of experiments all cultures were found to be infected with as many as 650 liver schizonts measuring up to 40 .mu.m. After the addition of red blood cells, intraerythrocytic forms of P. falciparum were detected on days 12 and 13 by an immunofluorescence assay, indicating that the hepatic cycle was completed.