Comparison of direct fluorescent antibody staining and real-time polymerase chain reaction for the detection of Borrelia burgdorferi in Ixodes scapularis ticks
Comparison of direct fluorescent antibody staining and real-time polymerase chain reaction for the detection of Borrelia burgdorferi in Ixodes scapularis ticks
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DOI：
10.1177/104063870601800610
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发表时间：
2006-11-01
影响因子：
1.5
通讯作者：
Bushmich, Sandra
中科院分区：
文献类型：
--
作者：
Gaumond, Gwenn;Tyropolis, Allison;Bushmich, Sandra
伯氏疏螺旋体是导致人类和动物莱姆病的病原体，通过受感染的硬蜱叮咬传播。滴答声。从人类和动物身上取出的蜱虫会由诊断实验室进行常规测试，以确定它们是否感染了这些细菌。本研究的目的是比较直接荧光抗体染色和实时聚合酶链反应 (PCR) 两种常用方法检测肩胛硬蜱中伯氏疏螺旋体的效果。对在莱姆病流行区康涅狄格州收集的 127 只成年肩胛蜱进行了测试，并对结果进行了比较。结果显示，24.8% 的蜱虫检测结果呈疏螺旋体阳性。通过荧光抗体检测，实时 PCR 检测显示 32.5% 的蜱呈伯氏疏螺旋体阳性。当蜱虫按充血程度（未充血、部分充血和完全充血）分组时，95% 的未充血蜱虫、90.5% 的部分充血蜱虫和 86.8% 的充血蜱虫测试结果一致。所检测的 127 只蜱虫中有 10 只脱水严重，无法通过荧光抗体技术进行检测；其中一半经 PCR 检测呈阳性。实时 PCR 似乎是这两种方法中诊断肩胛蜱细菌感染的更好方法。
Borrelia burgdoiferi, the agent responsible for causing Lyme disease in humans and animals, is transmitted via the bite of infected Ixodes spp. ticks. Ticks removed from humans and animals are routinely tested by diagnostic laboratories to determine if they are infected with these bacteria. The objective of this study was to compare the efficacy of 2 commonly used methods, direct fluorescent antibody staining and realtime polymerase chain reaction (PCR), for the detection of B. burgdorferi in Ixodes scapularis ticks. One hundred and twenty-seven adult I. scapularis ticks collected in Connecticut, a Lyme disease endemic area, were tested, and results were compared. Results showed 24.8% ticks tested positive for Borrelia spp. by fluorescent antibody testing and 32.5% ticks were positive for B. burgdorferi by real-time PCR testing. When ticks were grouped into categories by level of engorgement (unengorged, partially engorged, and fully engorged), 95% of unengorged ticks, 90.5% of partially engorged, and 86.8% of engorged ticks tested were in agreement. Ten of the 127 ticks examined were too dehydrated to be tested by the fluorescent antibody technique; half of these tested positive by PCR. Real-time PCR appears to be the better of these 2 methods for the diagnosis of this bacterial infection in I scapularis ticks.