Characterization and mapping of a salt-sensitive mutant in rice (Oryza sativa L.).
Characterization and mapping of a salt-sensitive mutant in rice (Oryza sativa L.).
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DOI：
10.1111/jipb.12048
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发表时间：
2013-06
影响因子：
11.4
通讯作者：
Jingwei Zhou;Fuzheng Wang;P. Deng;Wen Jing;Wenhua Zhang
中科院分区：
文献类型：
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作者：
Jingwei Zhou;Fuzheng Wang;P. Deng;Wen Jing;Wenhua Zhang
用甲基磺酸乙酯（EMS）诱变水稻品种日本晴的M2代，获得一个盐敏感突变体，命名为水稻盐敏感2号（rss 2）。该突变体的耐盐性下降，地上部Na+含量显著增加。遗传分析表明，rss 2中Na（+）含量的增加受一对隐性基因控制。对rss 2/窄叶青8号（ZYQ 8）F2群体构建的连锁图进行全基因组分析，发现位于第1和第6染色体上的2个数量性状位点（QTL）分别对地上部Na+浓度的表型变异贡献率为14.5%和53.3%。在Nipponbare/ZYQ 8的F2群体中也检测到了位于第1染色体上的QTL，而未检测到位于第6染色体上的QTL，表明位于第6染色体上的QTL可能是rss 2的盐敏感性基因。通过对rss 2/ZYQ 8扩大定位群体中220个突变个体的重组事件分析，将rss 2基因定位在插入/缺失（InDel）标记IM 21962和IM 22567之间605.3kb的区间内。这一发现将有助于rss 2位点的克隆，并为深入了解水稻盐敏感性的生理机制提供依据。
A salt-sensitive mutant designated rice salt sensitive 2 (rss2) was isolated from the M2 generation of the rice cultivar Nipponbare mutagenized with ethyl methanesulfonate (EMS). This mutant exhibited a greater decrease in salt tolerance with a significant increase in Na(+) content in its shoots. Genetic analysis indicated that the increase in Na(+) in rss2 was controlled by a single recessive gene. Further genome-wide analysis of the linkage map constructed from the F2 population of rss2/Zhaiyeqing 8 (ZYQ8) showed that two quantitative trait loci (QTLs) on chromosomes 1 and 6 were responsible for the Na(+) concentration in shoots, which explained 14.5% and 53.3%, respectively, of the phenotypic variance. The locus on chromosome 1, but not that on chromosome 6, was also detected in the F2 population of Nipponbare/ZYQ8, suggesting that the QTL on chromosome 6 was responsible for the salt sensitivity in rss2. By analyzing the recombination events in 220 mutant individuals of an enlarged mapping population of rss2/ZYQ8, the rss2 locus was precisely mapped to an interval of 605.3 kb between insertion/deletion (InDel) markers IM21962 and IM22567. This finding will facilitate the cloning of the rss2 locus and provide insight into the physiological mechanisms of salt sensitivity in rice.