ANALYSIS BY HIGH-RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS OF DIFFERENTIATION-DEPENDENT ALTERATIONS IN CYTOSOLIC PROTEIN PATTERN OF HL-60 LEUKEMIC-CELLS
ANALYSIS BY HIGH-RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS OF DIFFERENTIATION-DEPENDENT ALTERATIONS IN CYTOSOLIC PROTEIN PATTERN OF HL-60 LEUKEMIC-CELLS
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DOI：
10.1016/0165-022x(90)90044-d
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发表时间：
1990-06-01
影响因子：
--
通讯作者：
SEIFERT, R
中科院分区：
文献类型：
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作者：
JUNGBLUT, PR;SEIFERT, R
HL-60白血病细胞沿着嗜中性途径用维甲酸（RA）或沿着单核细胞途径用1，25-二羟维生素D3（1，25（OH）2D 3）分化。采用高分辨率双向电泳技术和随后的银染色，分析HL-60细胞的分化依赖性胞浆蛋白质模式的变化，并与人中性粒细胞胞浆蛋白质模式进行比较。与未分化的HL-60细胞相比，RA和1，25（OH）2D 3分化的HL-60细胞中研究的632种蛋白中有64种和50种蛋白的量分别增加或减少。其中33种蛋白质在RA和1，25（OH）2D 3分化的HL-60细胞中发生了类似的改变。与未分化的HL-60细胞相比，RA-或1，25（OH）2D 3-分化的HL-60细胞中22和25种蛋白质的量发生了变化，与未分化的HL-60细胞相比，人中性粒细胞中的蛋白质发生了类似的变化。在RA或1，25（OH）2D 3分化的HL-60细胞中，7种和10种蛋白质的量发生了变化，在中性粒细胞胞浆中具有特异性等价物。我们的研究结果表明：（I）嗜酸性粒细胞和单核细胞分化与胞质蛋白量的减少和增加有关;（ii）两种分化过程共有一组共同的改变;（iii）与蛋白量的特定改变有关。
HL-60 leukemic cells were differentiated along the neutrophilic pathway with retinoic acid (RA) or along the monocytic pathway with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). Using a high-resolution two-dimensional electrophoresis technique and subsequent silver staining, differentiation-dependent changes in cytosolic protein pattern of HL-60 cells were analyzed and were compared with the cytosolic protein pattern of human neutrophils. The amount of 64 and 50 out of a total of 632 proteins studied was increased or decreased in RA- and 1,25(OH)2D3-differentiated HL-60 cells, respectively, in comparison to undifferentiated HL-60 cells. Thirty-three of these proteins were similarly altered in RA- and 1,25(OH)2D3-differentiated HL-60 cells. Twenty-two and 25 of the proteins altered in amount in RA- or 1,25(OH)2D3-differentiated HL-60 cells versus undifferentated HL-60 cells were similarly altered in human neutrophils in comparison to undifferentiated HL-60 cells. Seven and 10 of the proteins altered in amount in RA- or 1,25(OH)2D3-differentiated HL-60 cells had specific equivalents in neutrophil cytosol. Our results show (I) that neutrophilic and monocytic differentiation is associated with decreases and increases in amount of cytosolic proteins; (ii) that both differentiation processes share a common set of alterations; and (iii) are associated with specific alterations in protein amount.