Thyroid hormone metabolism in cultured monkey hepatocarcinoma cells. Monodeiodination activity in relation to cell growth.
Thyroid hormone metabolism in cultured monkey hepatocarcinoma cells. Monodeiodination activity in relation to cell growth.
复制标题
培养猴肝癌细胞中的甲状腺激素代谢。
DOI：
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发表时间：
1980
影响因子：
4.8
通讯作者：
J. Robbins
中科院分区：
文献类型：
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作者：
K. Sato;J. Robbins
在单层猴肝癌细胞（NCLP-GE）中研究了甲状腺激素代谢，其中甲状腺素（T4）的主要代谢物是3,3',5'-三碘-L-甲状腺氨酸（e3），3,5,3'-三碘-~-甲状腺氨酸（T3）的主要代谢物是3,3'-二碘-~-甲状腺氨酸（3,3'-T~）和3'-单碘-~-甲状腺氨酸（3'-T1）。通过在缺乏异亮氨酸 (De(-)) 或异亮氨酸和谷氨酰胺 (ne(-)Gln(-)) 的培养基中培养来诱导细胞同步化。在这些生长停滞（主要是 G I 期）的细胞中，通过 lzBI 标记前体的非酚环脱碘的代谢途径（即 T4 转化为 r T 3 ，r T 3 转化为 3',5'-T~， T3 至 3,3'-T2 以及 3,3'-T2 至 3'-T1) 显着增加。还观察到 5' 脱碘（rT3 至 3,3'-T~）略有增加。使用含有二硫苏糖醇的细胞匀浆进行的 Lineweaver-Burk 分析显示，5'-脱碘（T4 至 rT3，0.059 PM，T3 至 3,3'-T4，0.034 PM）和 5'-脱碘（rT3 至 3,3'-T~）的表观 K,,, 3,3'-Tz, 1.3 厘米）
Thyroid hormone metabolism was studied in mono- layers of monkey hepatocarcinoma cells (NCLP-GE) in which the major metabolite of thyroxine (T4) is 3,3’,5’- triiodo-L-thyronine (e3), and those of 3,5,3’-triiodo-~-thyronine (T3) are 3,3’-diiodo-~-thyronine (3,3’-T~) and 3’-monoiodo-~-thyronine (3’-T1). Cells were induced to synchronize by culturing in medium deficient in isoleucine (De(-)) or in isoleucine and glutamine (ne(-)Gln(-)), In these growth-arrested (mainly G I phase) cells, the metabolic pathway through nonphenolic ring deiodination of lzBI-labeled precursors (namely, the conversion of T4 to r T 3 , r T 3 to 3’,5‘-T~, T3 to 3,3’-T2, and 3,3’-T2 to 3’-T1) was markedly increased. A slight increase in 5“deiodination (rT3 to 3,3‘-T~) was also observed. Lineweaver-Burk analysis using cell ho- mogenate containing dithiothreitol revealed that the apparent K,,, for 5-deiodination (T4 to rT3, 0.059 PM, and T3 to 3,3’-T4, 0.034 PM) and for 5’-deiodination (rT3 to 3,3’-Tz, 1.3 CM)