Molecular confirmation of natural hybridization between Lumnitzera racemosa and Lumnitzera littorea
Molecular confirmation of natural hybridization between Lumnitzera racemosa and Lumnitzera littorea
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Lumnitzera racemosa 和 Lumnitzera littorea 自然杂交的分子证实
DOI：
10.1016/j.aquabot.2011.03.001
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发表时间：
2011-07-01
期刊：
影响因子：
1.8
通讯作者：
Shi, Suhua
中科院分区：
文献类型：
--
作者：
Guo, Miaomiao;Zhou, Renchao;Shi, Suhua
20世纪70年代，根据形态特征，提出了印度-西太平洋地区两种红树林植物总状Lumnitzera racemosa和光叶Lumnitzera littorea的自然杂交假说;然而，没有分子证据支持它。在这项研究中，我们对两个低拷贝核基因和一个叶绿体基因间隔区进行了测序，（trnS-trnG）在两个Lumnitzera物种和他们的假定的杂交，以测试这一假设。结果表明，在同一单倍型间，两个核位点分别存在9个和27个核苷酸替换。总状花序和L littorea，和推定的混合显示在这些网站的色谱加和性。对两种Lumnitzera的叶绿体基因间区trnS-trnG进行测序，结果表明两种Lumnitzera在该区域存在7个固定的核苷酸替换和4个固定的插入/缺失，而推定的杂种与L.总状花序。分子生物学数据清楚地证明了L. racemosa和Littorea，L.总状花序是该杂交事件的母本。在这种情况下，杂交和F-1的杂交种的性质的不寻常的方向，并在红树林一般，进行了讨论。(C)2011爱思唯尔有限公司版权所有。
The hypothesis of natural hybridization between Lumnitzera racemosa and Lumnitzera littorea, two mangrove species distributed in the Indo-West Pacific region, was proposed in 1970s based on morphological traits; however, no molecular evidence has been reported to support it. In this study, we sequenced two low-copy nuclear genes and one chloroplast intergenic spacer (trnS-trnG) in the two Lumnitzera species and their putative hybrid to test this hypothesis. Our results revealed that there were 9 and 27 nucleotide substitutions at the two nuclear loci, respectively, between one haplotype of L. racemosa and L littorea, and that the putative hybrid showed additivity in chromatograms at these sites. Sequencing the chloroplast intergenic region trnS-trnG showed that the two Lumnitzera species differed by seven fixed nucleotide substitutions and four fixed insertions/deletions in this region, while the putative hybrid had identical sequences to L. racemosa. Molecular data clearly demonstrated that there indeed existed natural hybridization between L. racemosa and L littorea and that L. racemosa was the maternal parent in this hybridization event. The uncommon direction of hybridization and F-1 nature of hybrids in this case, and in mangroves in general, is discussed. (C) 2011 Elsevier B.V. All rights reserved.