THE STEREOCHEMICAL COURSE OF SULFURYL TRANSFER CATALYZED BY ARYLSULFATASE-II FROM ASPERGILLUS-ORYZAE
THE STEREOCHEMICAL COURSE OF SULFURYL TRANSFER CATALYZED BY ARYLSULFATASE-II FROM ASPERGILLUS-ORYZAE
复制标题
DOI：
10.1042/bj2870805
复制
发表时间：
1992-11-01
影响因子：
4.1
通讯作者：
LOWE, G
中科院分区：
文献类型：
--
作者：
CHAI, CLL;LOUGHLIN, WA;LOWE, G
合成了苯基硫酸盐[(R)-O-16,O-17,O-18]，研究了米曲霉芳基硫酸酶II催化硫基向对甲酚转移的立体化学过程。结果表明，该反应在硫原子上继续保持构型，这为硫酶中间体参与反应途径提供了证据。
Phenyl [(R)-O-16,O-17,O-18]sulphate was synthesized and used to study the stereochemical course of sulphuryl transfer to p-cresol catalysed by arylsulphatase II from Aspergillus oryzae. The reaction was shown to proceed with retention of configuration at the sulphur atom, providing evidence for the involvement of a sulpho-enzyme intermediate on the reaction pathway.