Differential sensitivity of specific and nonspecific antigen-presentation by B cells to a protein synthesis inhibitor.
Differential sensitivity of specific and nonspecific antigen-presentation by B cells to a protein synthesis inhibitor.
复制标题
B 细胞呈递的特异性和非特异性抗原对蛋白质合成抑制剂的敏感性不同。
作者：
T. Kakiuchi;M. Watanabe;N. Hozumi;H. Nariuchi
检查了B细胞的特异性和非特异性Ag呈递对用不可逆蛋白质合成抑制剂emperoxin治疗的敏感性。为此，将表达对TNP特异性的表面IgM受体的A20-HL B淋巴瘤细胞用作APC。OVA和TNP-OVA分别用作非特异性和特异性Ag。用雌二醇处理极大地削弱了A20-HL细胞将特异性Ag呈递给42-6A克隆的特异于OVA的T细胞的能力，但不影响非特异性Ag。艾美汀处理的A20-HL细胞呈递非特异性Ag的能力表明处理的细胞能够处理非特异性Ag并呈递处理的Ag。而A20-HL细胞通过表面受体对Ag的结合和内化不受雌二醇处理的影响。A20-HL细胞在放线菌酮（一种可逆的蛋白质合成抑制剂）的存在下摄取了用于刺激42-6A细胞的特异性Ag。这些结果表明，embryonic的行动是本地化的细胞内加工的特异性Ag，而不是非特异性Ag。因此，特异性Ag的加工途径似乎不同于非特异性Ag。
Specific and nonspecific Ag-presentation by B cells was examined for the sensitivity to the treatment with emetin, an irreversible protein synthesis inhibitor. For this aim, A20-HL B lymphoma cells expressing surface IgM receptors specific for TNP were used as APC. OVA and TNP-OVA were used as nonspecific and specific Ag, respectively. The treatment with emetin greatly impaired the ability of A20-HL cells to present specific Ag, but not nonspecific Ag, to 42-6A cloned T cells specific for OVA. The ability of the emetin-treated A20-HL cells to present nonspecific Ag indicates that the treated cells are able to process nonspecific Ag and to present processed Ag. Ag binding and the internalization by A20-HL cells through surface receptors were not affected by the emetin treatment. A20-HL cells took up specific Ag for stimulation of 42-6A cells in the presence of cycloheximide, a reversible protein synthesis inhibitor. These results suggest that the action of emetin is localized to the intracellular processing of specific Ag, not of nonspecific Ag. Thus, the processing pathway for specific Ag seems to be different from that for nonspecific Ag.