Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma.
Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma.
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发表时间：
2018-11
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通讯作者：
M. Lazarević;Maja S. Milošević;D. Trišić;Boško Toljić;Jelena Simonović;N. Nikolic;Nikola Mikovic
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作者：
M. Lazarević;Maja S. Milošević;D. Trišić;Boško Toljić;Jelena Simonović;N. Nikolic;Nikola Mikovic
最近的证据表明，一小群干细胞样细胞与肿瘤的发生、发展和转移有关。到目前为止，研究只集中在肿瘤内部这些细胞群的特征上，而忽略了肿瘤边缘，尽管已知肿瘤边缘的组织学和分子状态可能在疾病的过程中起着重要作用。因此，本研究的目的是从口腔鳞状细胞癌及其各自的边缘分离细胞，使用特定标记物对这些细胞进行表征，评估其自我更新潜力并确定其化疗耐药性。方法对12个组织标本（6个肿瘤和6个边缘）进行细胞培养。提取总RNA，采用实时荧光定量PCR （RT-PCR）分析基因表达。流式细胞术、免疫细胞术、成球法和MTT法也有应用。结果：来自肿瘤和肿瘤边缘的细胞均存在干细胞标记物CD133、Nanog、Sox2、CD44和Oct4，具有形成球状体的能力，并表现出化疗耐药性，差异较小。结论：边缘细胞亚群似乎具有干性，这可能会提出重新评估最佳手术处理的问题。
PURPOSE Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. METHODS Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RT-PCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. RESULTS With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. CONCLUSIONS Subpopulations of margin cells appeared to have stemness properties which might raise the question of re-evaluation of optimal surgical management.