Body Mass Index Differences in the Gut Microbiota Are Gender Specific.
Body Mass Index Differences in the Gut Microbiota Are Gender Specific.
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肠道微生物群的体重指数差异具有性别特异性
DOI：
10.3389/fmicb.2018.01250
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发表时间：
2018
影响因子：
5.2
通讯作者：
Hao Y
中科院分区：
文献类型：
--
作者：
Gao X;Zhang M;Xue J;Huang J;Zhuang R;Zhou X;Zhang H;Fu Q;Hao Y
背景：人们越来越认识到肠道微生物群在肥胖的发展中起着重要作用，但性别的影响仍然难以捉摸。我们的研究使用了大量的中国成年人队列，旨在确定肠道微生物群的差异作为体重指数（BMI）的函数，并研究这些差异中的性别特异性特征。方法：551名参与者根据体重指数被分为体重不足、正常、超重或肥胖。通过16S rRNA基因测序分析粪便微生物组组成。采用广义线性模型（GLM）、BugBase、PICRUSt和SPIEC-EASI来评估与BMI和性别相关的细菌群落丰富度、多样性、结构、生物水平微生物表型、分子功能和生态网络的变异性。结果：体重过轻组的细菌群落α多样性明显高于其他BMI组。当按性别分层时，女性的α多样性在BMI中保持不变，而男性的α多样性在BMI组之间没有显著差异。在肥胖雄性的粪便微生物群中观察到梭杆菌的富集，而肥胖雌性的粪便微生物群中放线菌的相对丰度增加。微生物群落表型分析显示，体重过轻的雄性往往有更多的厌氧细菌和更少的兼性厌氧细菌，这表明对氧化应激的抵抗力降低。在功能上，肥胖个体的丁酸-乙酰乙酸辅酶a转移酶富集，这可能有利于能量积累。研究发现，肥胖男性体内类似磷酸腺苷三磷酸酶增加，表明他们倾向于获取能量。肥胖组的微生物生态网络含有更多的拮抗微生物相互作用和高度节点。结论：通过一项大型中国队列研究，我们证明了bmi在肠道微生物群组成、功能和生态网络方面的相关差异，这些差异受性别的影响。这一领域的结果在几项独立研究中显示出差异，这表明需要进一步的研究来了解微生物群在调节宿主能量收集和储存中的作用，以及性别对这些功能的影响。
Background: The gut microbiota is increasingly recognized as playing an important role in the development of obesity, but the influence of gender remains elusive. Using a large cohort of Chinese adults, our study aimed to identify differences in gut microbiota as a function of body mass index (BMI) and investigate gender specific features within these differences. Methods: Five hundred fifty-one participants were categorized as underweight, normal, overweight, or obese, based on their BMI. Fecal microbiome composition was profiled via 16S rRNA gene sequencing. Generalized linear model (GLM), BugBase, PICRUSt, and SPIEC-EASI were employed to assess the variabilities in richness, diversity, structure, organism-level microbiome phenotypes, molecular functions, and ecological networks of the bacterial community that associated with BMI and sex. Results: The bacterial community of the underweight group exhibited significantly higher alpha diversity than other BMI groups. When stratified by gender, the pattern of alpha diversity across BMI was maintained in females, but no significant difference in alpha diversity was detected among the BMI groups of males. An enrichment of Fusobacteria was observed in the fecal microbiota of obese males, while obese females demonstrated an increased relative abundance of Actinobacteria. Analysis of microbial community-level phenotypes revealed that underweight males tend to have more anaerobic and less facultatively anaerobic bacteria, indicating a reduced resistance to oxidative stress. Functionally, butyrate-acetoacetate CoA-transferase was enriched in obese individuals, which might favor energy accumulation. PhoH-like ATPase was found to be increased in male obese subjects, indicating a propensity to harvest energy. The microbial ecological network of the obese group contained more antagonistic microbial interactions as well as high-degree nodes. Conclusion: Using a large Chinese cohort, we demonstrated BMI-associated differences in gut microbiota composition, functions, and ecological networks, which were influenced by gender. Results in this area have shown variability across several independent studies, suggesting that further investigation is needed to understand the role of the microbiota in modulating host energy harvest and storage, and the impact of sex on these functions.