The IMPROVE Guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments).
The IMPROVE Guidelines (Ischaemia Models: Procedural Refinements Of in Vivo Experiments).
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DOI：
10.1177/0271678x17709185
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发表时间：
2017-11
期刊：
影响因子：
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通讯作者：
Macrae IM
中科院分区：
文献类型：
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作者：
Percie du Sert N;Alfieri A;Allan SM;Carswell HV;Deuchar GA;Farr TD;Flecknell P;Gallagher L;Gibson CL;Haley MJ;Macleod MR;McColl BW;McCabe C;Morancho A;Moon LD;O'Neill MJ;Pérez de Puig I;Planas A;Ragan CI;Rosell A;Roy LA;Ryder KO;Simats A;Sena ES;Sutherland BA;Tricklebank MD;Trueman RC;Whitfield L;Wong R;Macrae IM
大多数缺血性卒中的体内模型靶向大脑中动脉，并且可以实现从轻度到严重的卒中严重程度谱。这篇综述描述了改善缺血性中风和动物福利的体内模型的机会。它提供了一些建议，以最大限度地降低最常见的啮齿动物大脑中动脉闭塞模型的严重程度，同时维持或改善科学结果。这些建议包括术前的基本要求，选择最合适的麻醉和镇痛方案，以及术中和术后护理。其目的是为研究人员和动物护理人员提供支持，以完善他们的程序和实践，并实施小的增量变化，以改善所使用动物的福利，并回答正在调查的科学问题。所有建议都概括在一张摘要海报中（见补充资料）。
Most in vivo models of ischaemic stroke target the middle cerebral artery and a spectrum of stroke severities, from mild to substantial, can be achieved. This review describes opportunities to improve the in vivo modelling of ischaemic stroke and animal welfare. It provides a number of recommendations to minimise the level of severity in the most common rodent models of middle cerebral artery occlusion, while sustaining or improving the scientific outcomes. The recommendations cover basic requirements pre-surgery, selecting the most appropriate anaesthetic and analgesic regimen, as well as intraoperative and post-operative care. The aim is to provide support for researchers and animal care staff to refine their procedures and practices, and implement small incremental changes to improve the welfare of the animals used and to answer the scientific question under investigation. All recommendations are recapitulated in a summary poster (see supplementary information).