Methylation Status of Lamin A/C in Gastric Cancer Cell Lines
Methylation Status of Lamin A/C in Gastric Cancer Cell Lines
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DOI：
10.5754/hge11610
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发表时间：
2012-06-01
影响因子：
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通讯作者：
Rha, Sun Young
中科院分区：
文献类型：
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作者：
Lee, Won Suk;Jung, Jae Joon;Rha, Sun Young
背景/目的：表观遗传调控在癌症的发生发展中发挥重要作用。因此，发现新的表观遗传调控基因可以为了解癌症提供有用的信息。Lamin A/C是一种中间纤维蛋白，据报道其在胃肠道恶性肿瘤组织中的表达受到抑制。我们检测了纤层蛋白A/C在胃癌和结直肠癌细胞系中的表达及其与DNA甲基化的关系。方法：采用甲基化特异性PCR方法，对19例胃癌细胞、5例结直肠癌细胞和1例正常结肠癌细胞的CpG岛甲基化状态进行检测。采用RT-PCR检测层粘胶蛋白A/C mRNA表达水平。结果：18株胃癌细胞株层粘连蛋白A/C未甲基化95%，1株部分甲基化。在结直肠癌中，5个癌细胞系中只有1个（20%）部分甲基化，其余细胞系，包括1个正常结肠细胞系未甲基化。RT-PCR显示，无论甲基化状态如何，所有细胞系均表现出层粘连蛋白A/C的mRNA表达。结论：我们观察到，不同于血液恶性细胞，胃肠道肿瘤细胞系中lamin A/C的表达不受抑制，也不受DNA甲基化的调节。
Background/Aims: Epigenetic regulations play a role in the development and progression of cancer. Therefore, discovering novel epigenetically regulated genes could provide useful information in understanding cancer. Lamin A/C is an intermediate filament protein whose expression is reported to be suppressed in tissues of gastro-intestinal malignancies. We examined expression of lamin A/C in gastric and colorectal cancer cell lines and its association with DNA methylation. Methodology: The methylation status of CpG island in 19 gastric, 5 colorectal cancer cells and 1 normal colon cell line were examined with methylation-specific PCR using paired methylated and unmethylated primers. The level of mRNA expression of lamin A/C was detected using RT-PCR. Results: Eighteen gastric cancer cell lines showed 95% unmethylation of lamin A/C and 1 cell line showed partial methylation. In colorectal cancer, only 1 out of 5 cancer cell lines (20%) was partially methylated and the remaining cell lines, including 1 normal colon cell line was unmethylated. With RT-PCR, all cell lines demonstrated mRNA expression of lamin A/C regardless of methylation status. Conclusions: We observed that the expression of lamin A/C was not suppressed in gastrointestinal cancer cell lines different from hematologic malignant cells and it is not regulated through DNA methylation.