Effects of adsorbed heat labile serum proteins and fibrinogen on adhesion and apoptosis of monocytes/macrophages on biomaterials
Effects of adsorbed heat labile serum proteins and fibrinogen on adhesion and apoptosis of monocytes/macrophages on biomaterials
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DOI：
10.1023/a:1024951330265
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发表时间：
2003-08-01
影响因子：
3.7
通讯作者：
Anderson, JM
中科院分区：
文献类型：
--
作者：
Brodbeck, WG;Colton, E;Anderson, JM
先前建立的人单核细胞培养方案用于确定不同吸附蛋白对单核细胞/巨噬细胞粘附和存活在二甲基硅烷（DM）或RGD修饰的玻璃盖玻片上的影响。使细胞在不存在蛋白质或存在血清、纤维蛋白原（Fg）、热灭活血清（HIS）、补充有Fg的血清或HIS与Fg的情况下粘附2小时。在培养的第0天（2 h）、第3天、第7天和第10天测定细胞粘附率和细胞凋亡率。初始培养物中仅存在血清足以优化单核细胞/巨噬细胞粘附和存活率。加入Fg血清既不增加粘附也不降低凋亡率。没有蛋白质或添加HIS在初始孵育期间显着降低单核细胞/巨噬细胞的粘附和存活在两个表面上，然而，添加Fg到HIS恢复粘附和存活率，在单独的血清存在下观察到的那些在RGD表面上。这些研究表明，单核细胞/巨噬细胞的粘附和生物材料表面上的生存优化吸附的热不稳定血清蛋白，而吸附的纤维蛋白原起着表面性质依赖性的作用。(C)2003 Kluwer学术出版社
A previously established human monocyte culture protocol was used to determine the effects of varying adsorbed proteins on monocyte/macrophage adhesion and survival on dimethyl-silane (DM) or RGD modified glass coverslips. Cells were allowed to adhere for 2 h in the absence of protein or in the presence of serum, fibrinogen (Fg), heat inactivated serum (HIS), serum supplemented with Fg or HIS with Fg. Cell adhesion and apoptosis rates were determined on days 0 (2 h), 3, 7 and 10 of culture. The presence of serum alone in the initial culture was sufficient to optimize monocyte/macrophage adhesion and survival rates. Adding Fg to serum did not increase adhesion nor decrease apoptotic rates. No protein or the addition of HIS during the initial incubation period significantly decreased monocyte/ macrophage adhesion and survival on both surfaces, however, the addition of Fg to HIS restored adhesion and survival rates to those seen with in the presence of serum alone on RGD surfaces. These studies demonstrate that monocyte/macrophage adhesion and survival on biomaterial surfaces are optimized by adsorbed heat labile serum proteins while adsorbed Fg plays a surface property-dependent role. (C) 2003 Kluwer Academic Publishers