New derivatization reagents for the resolution of amino acid enantiomers by high-performance liquid chromatography
New derivatization reagents for the resolution of amino acid enantiomers by high-performance liquid chromatography
复制标题
用于高效液相色谱拆分氨基酸对映体的新型衍生试剂
DOI：
10.1016/s0021-9673(00)85077-3
复制
发表时间：
1978
影响因子：
4.1
通讯作者：
T. Nambara
中科院分区：
文献类型：
--
作者：
J. Goto;M. Hasegawa;Setsuko Nakamura;K. Shimada;T. Nambara
建立了一种将对映体胺转化为非对映异构体的新的衍生化方法，并用高效液相色谱进行拆分。合成了两种手性试剂(−)-a-甲氧基-a-甲基-1-萘乙酸和(−)-a-甲氧基-a-甲基-2-萘乙酸，并通过其(+)-α-甲基苄胺盐的分离结晶进行了光学拆分。用N，N‘-二环己基碳二亚胺法将氨基酸甲酯与(−)-a-甲氧基-a-甲基-1-萘乙酸制得的非对映异构体在正相柱上得到有效的拆分，并在紫外检测器上获得了令人满意的灵敏度。
A new derivatization procedure has been developed for converting enantiomeric amines into diastereomers for resolution by high-performance liquid chromatography. Two chiral reagents, (−)-a-methoxy-a-methyl-1-naphthaleneacetic acid and (−)-a-methoxy-a-methyl-2-naphthaleneacetic acid, were prepared and optically resolved by fractional crystallization of their (+)-a-methylbenzylamine salts. The diastereomeric amides formed from amino acid methyl esters and (−)-a-methoxy-a-mthyl-1-naphthaleneacetic acid by the N,N′-dicyclohexylcarbodiimide method were efficiently resolved on a normal-phase column and responded with satisfactory sensitivity in the ultraviolet detector.