Lipid and fatty acid profile of the retina, retinal pigment epithelium/choroid, and the lacrimal gland, and associations with adipose tissue fatty acids in human subjects
Lipid and fatty acid profile of the retina, retinal pigment epithelium/choroid, and the lacrimal gland, and associations with adipose tissue fatty acids in human subjects
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DOI：
10.1016/j.exer.2008.08.010
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发表时间：
2008-12-01
影响因子：
3.4
通讯作者：
Creuzot-Garcher, Catherine P.
中科院分区：
文献类型：
--
作者：
Bretillon, Lionel;Thuret, Gilles;Creuzot-Garcher, Catherine P.
布鲁赫膜（Bruch’s membrane，BrM）内以及BrM和视网膜色素上皮（retinal pigment epithelium，RPE）之间的脂质积累是与正常衰老相关的生物学变化之一，并且可能有助于年龄相关性黄斑病变的发展。这些脂质的来源仍在积极研究中。血浆脂质和来自神经视网膜的脂质的相对贡献仍然存在争议。低密度脂蛋白（LDL）已被报道显着参与视网膜的脂质供应后，积极重塑内的RPE。同时，RPE表达合成脂蛋白样颗粒的酶机制。本研究的目的是建立眼部结构和脂肪组织的脂肪酸谱之间的关联，作为受试者过去饮食摄入量的替代。从年龄为59-95岁的27名人类供体（19名女性，8名男性）收集的神经视网膜、视网膜色素上皮（RPE）/脉络膜、泪腺和脂肪组织中分析脂质和脂肪酸。神经视网膜中的DNA浓度与来自RPE/脉络膜的胆固醇酯（CE）的浓度正相关，并且与来自RPE/脉络膜的磷脂（PL）中的DHA浓度负相关。眼眶脂肪中的DHA与泪腺中的DHA呈正相关。在其他眼部结构中未观察到显著相关性。眼眶脂肪中的亚油酸与泪腺中的亚油酸正相关，其次是神经视网膜和来自RPE/脉络膜的CE;它与来自RPE/脉络膜的PL略有相关性。在眼眶脂肪、泪腺、PL和来自RPE/脉络膜的CE中检测到完全源自饮食的其他脂肪酸，例如反式脂肪酸。与其他脂肪酸如亚油酸相反，神经视网膜中的DHA与其膳食摄入量的相关性很差。在这种情况下，CE可能是进入视网膜的脂肪酸的重要载体。尽管流行病学研究报告了DHA在预防年龄相关性黄斑变性（西方国家失明的主要原因）方面的益处，但补充DHA的患者的相关性受到质疑。(C)2008爱思唯尔有限公司保留所有权利。
Accumulation of lipids within Bruch's membrane (BrM) and between BrM and retinal pigment epithelium (RPE) accounts for one of the biological changes associated with normal aging and may contribute to the development of age-related maculopathies. The origin of these lipids is still being actively investigated. The relative contribution of plasma lipids and lipids coming from the neural retina remains a matter of controversy. Low-density lipoproteins (LDLs) have been reported to significantly participate in the retina's lipid supply, after active remodeling within RPE. Meanwhile, RPE expresses the enzymatic machinery for synthesizing lipoprotein-like particles. The objective of this study was to establish associations between the fatty acid profile of the ocular structures and adipose tissue as a surrogate for the subjects' past dietary intake. Lipids and fatty acids were analyzed from the neural retina, retinal pigment epithelium (RPE)/choroid, the lacrimal gland, and adipose tissue, collected from 27 human donors (19 women, eight men) aged 59-95 years. DNA concentrations in the neural retina were positively associated with the concentrations in cholesteryl esters (CEs) from RPE/choroid and negatively associated with DHA concentrations in phospholipids (PLs) from RPE/choroid. DHA in orbital fat was positively associated with DHA in the lacrimal gland. No significant association was observed in the other ocular structures. Linoleic acid in orbital fat was positively associated with linoleic acid in the lacrimal gland, followed by the neural retina and CEs from RPE/choroid; it was slightly correlated with PLs from RPE/choroid. Other fatty acids that originate exclusively from the diet such as trans fatty acids were detected in orbital fat, the lacrimal gland, PLs, and CEs from RPE/choroid. DHA in the neural retina was poorly associated with its dietary intake, contrary to other fatty acids such as linoleic acid. Within this context, CEs may be important carriers of fatty acids entering the retina. Although epidemiological studies have reported the benefit of DHA in the prevention of age-related macular degeneration, the leading cause of blindness in Western countries, the relevance of supplementing patients with DHA is questioned. (C) 2008 Elsevier Ltd. All rights reserved.