Distribution of insensitive acetylcholinesterase (ace-1R) in Anopheles gambiae s.l. populations from Burkina Faso (West Africa)
Distribution of insensitive acetylcholinesterase (ace-1R) in Anopheles gambiae s.l. populations from Burkina Faso (West Africa)
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DOI：
10.1111/j.1365-3156.2009.02243.x
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发表时间：
2009-04-01
影响因子：
3.3
通讯作者：
Baldet, T.
中科院分区：
文献类型：
--
作者：
Dabire, K. R.;Diabate, A.;Baldet, T.
通过在布基纳法索三个不同农业气候带的20个地点进行全国范围的抽样调查，了解乙酰胆碱酯酶不敏感突变ace-1（R）的分布情况，该突变可使安氏按蚊对有机磷（OP）和氨基甲酸酯（CM）杀虫剂产生抗药性。2006年8月至10月，通过室内喷雾收集成蚊。采用聚合酶链反应（PCR）技术对采集的标本进行种属鉴定，并采用PCR-限制性片段长度多态性技术对ace-1（R）突变进行鉴定。冈比亚公司和一个.苏丹-萨赫勒（78.6%vs.21.4%）和萨赫勒（91.5%vs.8.5%）生态类型中的阿拉伯藜（98.3%vs.1.7%）。安。冈比亚S型在西部苏丹地区占主导地位（69%对31%的M型），但在萨赫勒地区未发现（100% M型）。ace-1（R）突变以中等频率（< 50%）分散在苏丹和苏丹-萨赫勒地区，但在萨赫勒地区不存在。它在S型蚊子中比M型蚊子更普遍（S型为0.32对M型为0.036）。没有安。检测到阿拉伯人携带突变。ace-1（R）在苏丹和苏丹-萨赫勒地区的地理分布与分布在这两个气候带的棉花种植区相关，这些结果具有特殊意义，因为OP和CM杀虫剂已被提议作为目前仅用于许多病媒控制方案的拟除虫菊酯的替代品或添加剂。
To investigate through countrywide sampling at 20 localities across the three different agro-climatic zones of Burkina Faso, the distribution of the acetylcholinesterase insensitive mutation ace-1(R), which confers resistance to organophosphates (OP) and carbamates (CM) insecticides in An. gambiae s.l.Adult mosquitoes were collected by indoor aerosol spraying from August to October 2006. Specimens were identified to species by polymerase chain reaction (PCR) assay and characterized for the ace-1(R) mutation using a PCR-restriction fragment length polymorphism diagnostic.Collected mosquitoes were a mixture of An. gambiae s.s. and An. arabiensis across the Sudan (98.3%vs. 1.7%), Sudan-sahelian (78.6%vs. 21.4%) and the Sahel (91.5%vs. 8.5%) ecotypes. The An. gambiae S-form predominated in the Sudan sites from the West (69%vs. 31% for the M form) but was not found in the Sahel (100% M form). The ace-1(R) mutation was dispersed throughout the Sudan and Sudan-sahelian localities at moderate frequency (< 50%) but was absent in the Sahel. It was far more prevalent in S form than M form mosquitoes (0.32 for the S form vs. 0.036 for the M form). No An. arabiensis was detected carrying the mutation. The geographic distribution of ace-1(R) in the Sudan and Sudan-sahelian correlated with the cotton growing areas dispersed throughout the two climatic zones.These results have special significance as OP and CM insecticides have been proposed as alternatives or additions to pyrethroids which are currently used exclusively in many vector control programmes.