A HISTOCHEMICAL-STUDY OF IRON, TRANSFERRIN, AND FERRITIN IN ALZHEIMERS DISEASED BRAINS
A HISTOCHEMICAL-STUDY OF IRON, TRANSFERRIN, AND FERRITIN IN ALZHEIMERS DISEASED BRAINS
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DOI：
10.1002/jnr.490310111
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发表时间：
1992-01-01
影响因子：
4.2
通讯作者：
MUFSON, EJ
中科院分区：
文献类型：
--
作者：
CONNOR, JR;MENZIES, SL;MUFSON, EJ
对尸检获得的阿尔茨海默病脑组织进行免疫组织化学和组织化学染色。铁调节蛋白转铁蛋白和铁蛋白以及铁，在一般情况下，主要发现在少突胶质细胞类似于以前报道的正常脑组织。然而，在老年斑附近，蛋白质和铁的染色模式发生改变。转铁蛋白均匀分布在老年斑周围，明显位于细胞外。此外，在阿尔茨海默病组织的大脑皮质白色物质中的星形胶质细胞中发现转铁蛋白，而不是其在少突胶质细胞中的正常分布。一个强大的铁蛋白免疫反应伴随着老年斑和许多血管在阿尔茨海默氏症的脑组织。虽然许多铁蛋白阳性的少突胶质细胞存在于阿尔茨海默病组织中，但大多数与老年斑和血管相关的含铁蛋白的细胞是小胶质细胞。铁也可以在老年斑中表现出来。铁反应产物在斑块附近和与斑块相关的细胞中均弥漫性观察到。这些数据强烈表明，在阿尔茨海默氏病的脑铁稳态的破坏所证明的铁和负责铁调节的蛋白质的正常细胞分布的改变。这些数据将有助于了解阿尔茨海默病中氧化损伤的潜力和金属神经毒性的潜力。
Immunohistochemical and histochemical staining were performed on Alzheimer's diseased brain tissue obtained at autopsy. The iron-regulatory proteins transferrin and ferritin as well as iron are, in general, found predominantly in oligodendrocytes similar to that previously reported for normal brain tissue. However, in the vicinity of senile plaques, the staining pattern is altered for both proteins and iron. Transferrin is homogenously distributed around the senile plaques and is apparently extracellular. In addition, transferrin is found in astrocytes in the cerebral cortical white matter of the Alzheimer's tissue rather than its normal distribution in oligodendrocytes. A robust ferritin immunoreaction accompanies senile plaques and many blood vessels in the Alzheimer's brain tissue. Although many ferritin-positive oligodendrocytes are present in the Alzheimer's tissue, most of the ferritin-containing cells associated with senile plaques and blood vessels are microglia. Iron can also be demonstrated in the senile plaques. The iron reaction product is observed both diffusely in proximity of the plaques and in cells associated with the plaques. These data strongly suggest a disruption in brain iron homeostasis in Alzheimer's disease as demonstrated by alterations in the normal cellular distribution of iron and the proteins responsible for iron regulation. These data will contribute to understanding both the potential for oxidative damage and the potential for metal neurotoxicity in Alzheimer's disease.