Effect of technetium-99 conjugated with methylene diphosphonate (Tc-99-MDP) on OPG/RANKL/RANK system in vitro
Effect of technetium-99 conjugated with methylene diphosphonate (Tc-99-MDP) on OPG/RANKL/RANK system in vitro
复制标题
锝-99与亚甲基二膦酸酯（Tc-99-MDP）缀合对体外OPG/RANKL/RANK系统的影响
DOI：
10.1111/jop.12801
复制
发表时间：
2019
影响因子：
3.3
通讯作者：
Qian Yemei
中科院分区：
文献类型：
--
作者：
Shen Shiying;Wang Weihong;Yang Chun;Xu Biao;Zeng Ling;Qian Yemei
RANKL和RANK在成釉细胞瘤的发生发展过程中对颌骨吸收起重要作用。因此，本研究旨在探讨99 Tc-MDP对体外培养的RAW264.7和MC 3 T3-E1细胞OPG/RANKL/RANK系统的影响，为临床治疗颌骨成釉细胞瘤提供理论依据。CCK-8法检测细胞增殖抑制率。流式细胞仪检测细胞凋亡和细胞周期。结果不同浓度的99 Tc-MDP对RAW 264. 7细胞株均有抑制作用，RANK蛋白表达降低。除100 μg/mL浓度组外，99 Tc-MDP对MC 3 T3-E1细胞的增殖作用在48 h时均强于24 h时。与0.01 μg/mL浓度相比，100 μg/mL 99 Tc-MDP处理MC 3 T3-E1细胞，24 h和48 h细胞增殖效应降低（P<0.05）。经0.01 μg/mL 99 Tc-MDP处理后，MC 3 T3-E1细胞OPG表达明显增加（P<0.05）。结论99 Tc-MDP可诱导RAW 264. 7细胞凋亡，并抑制RANK蛋白的表达。0.01 μg/mL低浓度的99 Tc-MDP可促进MC 3 T3-E1细胞增殖，增加OPG和RANKL蛋白的表达，对颌骨成釉细胞瘤的治疗可能具有辅助治疗作用。
BackgroundRANKL and RANK play an important role in jaw resorption during the development of the ameloblastomas. Therefore, the aim of this study was to explore the effect of99Tc‑MDP on OPG/RANKL/RANK system on RAW264.7 and MC3T3‐E1 cell lines in vitro and provide the theoretical basis for the clinical treatment of the jaw ameloblastoma.MethodsDifferent concentrations of99Tc‐MDP were used to treat RAW264.7 and MC3T3‐E1 cell lines. The cell proliferative inhibition rate was analyzed by CCK‐8. Cell apoptosis and cell cycle were detected by flow cytometry. Western blot was used to detect the expression of OPG, RANKL, and RANK.ResultsTreatment of RAW264.7 cell lines with different concentrations of99Tc‐MDP had inhibitory effects and decreased the expression of RANK protein. The cell proliferation of99Tc‐MDP on MC3T3‐E1 cell lines was stronger at 48 hours than at 24 hours except for 100 μg/mL concentration group. Compared with the concentration of 0.01 μg/mL, the treatment of MC3T3‐E1 cells with 100 μg/mL99Tc‐MDP showed that the cell proliferative effect decreased at 24 hours and 48 hours (P<0.05). After treatment with 0.01 μg/mL99Tc‐MDP, the expression of OPG in MC3T3‐E1 cells was significantly increased (P<0.05). Compared with 0.01 μg/mL, the expression of RANKL was decreased after treatment with 100 μg/mL99Tc‐MDP (P<0.05).Conclusion99Tc‐MDP can induce apoptosis of RAW264.7 cells and inhibit the expression of RANK protein. The effect of 0.01 μg/mL of low concentration of99Tc‐MDP can promote the proliferation of MC3T3‐E1 cells and increase the expression of OPG and RANKL protein.99Tc‐MDP may have adjuvant therapeutic effects on the treatment of jaw ameloblastoma.