Conserved mechanism for vacuolar magnesium sequestration in yeast and plant cells
Conserved mechanism for vacuolar magnesium sequestration in yeast and plant cells
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DOI：
10.1038/s41477-021-01087-6
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发表时间：
2022-01
期刊：
影响因子：
18
通讯作者：
R. Tang;Sufang Meng;Xiaojiang Zheng;Binbin Zhang;Yang Yang-Yang;Chao Wang;Aigen Fu;Fugeng Zhao;Wenzhi Lan;S. Luan
中科院分区：
文献类型：
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作者：
R. Tang;Sufang Meng;Xiaojiang Zheng;Binbin Zhang;Yang Yang-Yang;Chao Wang;Aigen Fu;Fugeng Zhao;Wenzhi Lan;S. Luan
镁是所有生命形式所必需的营养物质。在真菌和植物细胞中，大部分的镁离子储存在液泡中，但镁离子转运到液泡库的机制还不完全清楚。在这里，我们证明了来自酿酒酵母和拟南芥的古老保守结构域蛋白(ACDPs)在液泡镁离子隔离中的功能，使植物和酵母细胞能够应对高水平的外部镁离子。我们发现酵母基因组(以及其他真菌基因组)含有单一的ACDP同源物，称为MAM3，它在液泡中积累镁离子方面具有特殊的功能，是耐受高镁所必需的。同时，从拟南芥中鉴定出了空泡型ACDP同源物，并证明它可以补充酵母突变株MAN3Δ。与野生型相比，缺乏一个液泡ACDP同源物的拟南芥突变体表现出对高镁条件的敏感，并且在液泡中积累的镁较少。综上所述，我们的结果表明，保守的转运蛋白介导了真菌和植物细胞中液泡镁离子的封存，以维持细胞内镁离子的动态平衡，以响应环境中镁离子水平的波动。
Magnesium (Mg2+) is an essential nutrient for all life forms. In fungal and plant cells, the majority of Mg2+is stored in the vacuole but mechanisms for Mg2+transport into the vacuolar store are not fully understood. Here we demonstrate that members of ancient conserved domain proteins (ACDPs) fromSaccharomyces cerevisiaeandArabidopsis thalianafunction in vacuolar Mg2+sequestration that enables plant and yeast cells to cope with high levels of external Mg2+. We show that the yeast genome (as well as other fungal genomes) harbour a single ACDP homologue, referred to as MAM3, that functions specifically in vacuolar Mg2+accumulation and is essential for tolerance to high Mg. In parallel, vacuolar ACDP homologues were identified fromArabidopsisand shown to complement the yeast mutantmam3Δ. AnArabidopsismutant lacking one of the vacuolar ACDP homologues displayed hypersensitivity to high-Mg conditions and accumulated less Mg in the vacuole compared with the wild type. Taken together, our results suggest that conserved transporters mediate vacuolar Mg2+sequestration in fungal and plant cells to maintain cellular Mg2+homeostasis in response to fluctuating Mg2+levels in the environment.