A thermostable transketolase evolved for aliphatic aldehyde acceptors.
A thermostable transketolase evolved for aliphatic aldehyde acceptors.
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DOI：
10.1039/c4cc08436e
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发表时间：
2015-01
影响因子：
4.9
通讯作者：
D. Yi;T. Saravanan;T. Devamani;F. Charmantray;L. Hecquet;W. Fessner
中科院分区：
文献类型：
--
作者：
D. Yi;T. Saravanan;T. Devamani;F. Charmantray;L. Hecquet;W. Fessner
基于智能文库的ph比色筛选，对嗜热硬脂地杆菌的热稳定转酮醇酶进行定向进化，产生了几个突变体，在不对称碳化步骤中，它们对脂肪醛的活性高达16倍，对着体选择性高（>95% ee）。
Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95% ee) in the asymmetric carboligation step.