INDEPENDENT REGULATION OF COLLAGEN TYPES BY CHONDROCYTES DURING THE LOSS OF DIFFERENTIATED FUNCTION IN CULTURE
INDEPENDENT REGULATION OF COLLAGEN TYPES BY CHONDROCYTES DURING THE LOSS OF DIFFERENTIATED FUNCTION IN CULTURE
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DOI：
10.1016/0092-8674(78)90056-9
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
NIMNI, ME
中科院分区：
文献类型：
--
作者：
BENYA, PD;PADILLA, SR;NIMNI, ME
测定兔关节软骨细胞在软骨切片和第1~8单层培养中的胶原表型。在切片培养的前24 h，软骨细胞表现为96%的II型、3%的X2Y和1%的III型。在原代单层培养中，这种分化的软骨细胞表型没有添加其他类型的胶原。每个表达的胶原蛋白的单位细胞合成都被刺激。原代培养第5天，X2Y合成量增加10倍，第8天进一步增加4倍。而III型胶原的合成在第5天无明显变化，第8天增加了7倍。建议在这种情况下对X2Y进行独立调控。在一个单独的实验中，研究了第一-第五种培养物。在第5代培养时，II型胶原的合成/细胞数稳步下降，基本停止，表明这些软骨细胞的后代丧失了分化功能。在第3代培养中首次检测到I型三聚体和I型胶原的合成，但II型合成的缺失不能定量地被I型三聚体和I型胶原的合成所取代。在这些表型质变发生的同时，III型胶原合成的刺激率没有改变，X2Y的合成刺激率仅略有下降。因此，II型合成的终止并没有显著改变分化的软骨细胞产生的其他胶原蛋白的合成。最终的去分化表型为I型41%，X2Y25%，I型三聚体20%，III型13%，II型1%。
Collagen phenotypes were determined for rabbit articular chondrocytes in cartilage slices and 1st - 8th monolayer cultures. During the 1st 24 h of slice culture, chondrocytes exhibited the following collagen phenotype: 96% type II, 3% X2Y and 1% type III. In primary monolayer culture, no other types of collagen were added to this differentiated chondrocyte phenotype. The synthesis per cell of each of the expressed collagens was stimulated. By the 5th day of primary culture X2Y synthesis increased 10 X, and by the 8th day, a further 4-fold. In contrast, the synthesis of collagen types II and III showed no change by the 5th day, but increased 7-fold by the 8th day. Independent regulation of X2Y in this situation was suggested. In a separate experiment 1st-5th cultures were studied. The synthesis/cell of type II colagen declined steadily and essentially ceased by the 5th culture, indicating the loss of differentiated function by these chondrocyte progeny. The loss of type II synthesis was not quantitatively replaced by the synthesis of type I trimer and type I collagen which was 1st detected in the 3rd culture. While these qualitative changes in phenotype occurred, the stimulated rate of type III collagen synthesis did not change and that of X2Y declined only slightly. Thus the termination of type II synthesis did not significantly alter the synthesis of the other collagens produced by differentiated chondrocytes. The final de-differentiated phenotype was 41% type I, 25% X2Y, 20% type I trimer, 13% type III and 1% type II.