Chromosomal Regions Underlying Noncoagulation of Milk in Finnish Ayrshire Cows
Chromosomal Regions Underlying Noncoagulation of Milk in Finnish Ayrshire Cows
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DOI：
10.1534/genetics.107.083964
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发表时间：
2008-10-01
期刊：
影响因子：
3.3
通讯作者：
Ojala, Matti
中科院分区：
文献类型：
--
作者：
Tyriseva, Anna-Maria;Elo, Kari;Ojala, Matti
大约 10% 的芬兰艾尔郡奶牛生产非凝固奶，即在标准 30 分钟测试时间内不会形成凝乳的牛奶，因此是奶酪乳制品的不良原料。这种现象与泌乳高峰和中期有关，但有些奶牛持续产出不凝固的奶。采用选择性 DNA 合并方法进行全基因组扫描，以定位与乳汁不凝固相关的基因组区域。基于相同历史突变的假设，我们汇集了不同种牛的数据。在测试池的同质性之前，针对 PCR 伪影（即阴影带和差异扩增）校正等位基因强度。使用子代设计和家族内的选择性基因分型验证了表明关联的结果。数据包括 18 个公牛家系，总共 477 个基因分型的女儿，即每尾的凝乳能力的 12%。使用区间映射tinder最大似然法和非参数方法对数据进行分析。 2 号染色体上的 BMS1126 和 18 号染色体上的 BMS1355 与不同家庭的乳汁不凝固相关，实验显着性水平为 0.1%。通过扫描基因数据库，我们发现了两个潜在的候选基因：LOC538897（2号染色体上的一种非特异性丝氨酸/苏氨酸激酶）和SIAT4B（一种唾液酸转移酶，催化18号染色体上K-酪蛋白糖基化的最后一步）。显然需要进一步研究以确定候选基因在牛奶不凝固中的作用。
About 10% of Finnish Ayrshire cows produce noncoagulating milk, i.e., milk that does not form a curd in a standard 30-min testing time and is thus a poor raw material for cheese dairies. This phenomenon is associated with peak and midlactation, but Some cows produce noncoagulating milk persistently. A genomewide scan under a selective DNA pooling method was carried Out to locate genomic regions associated with the noncoagulation of milk. On the basis of the hypothesis of the same historical mutation, we pooled the data across sires. Before testing pools for homogeneity, allele intensities were corrected for PCR artifacts, i.e., shadow bands and differential amplification. Results indicating association were verified using daughter design and selective genotyping within families. Data consisted of 18 sire families with 477 genotyped daughters in total, i.e., 12% of each tail of the milk coagulation ability. Data were analyzed using interval mapping tinder maximum-likelihood and nonparametric methods. BMS1126 on chromosome 2 and BMS1355 on chromosome 18 were associated with noncoagulation of milk across families on an experimentwise 0.1% significance level. By scanning gene databases, we found two potential candidate genes: LOC538897, a nonspecific serine/threonine kinase on chromosome 2, and SIAT4B, a sialyl transferase catalyzing the last step of glycosylation Of K-casein on chromosome 18. Further studies to determine the role of the candidates in the noncoagulation of milk are clearly needed.