Attenuation of endothelial-dependent vasodilator responses, induced by dye-encapsulated silica nanoparticles, in aortic vessels.
Attenuation of endothelial-dependent vasodilator responses, induced by dye-encapsulated silica nanoparticles, in aortic vessels.
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DOI：
10.2217/nnm.12.213
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发表时间：
2014-04
期刊：
影响因子：
5.5
通讯作者：
Asima Farooq;Debra Whitehead;M. Azzawi
中科院分区：
文献类型：
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作者：
Asima Farooq;Debra Whitehead;M. Azzawi
目的探讨纳米二氧化硅颗粒（SiNP）的数量、大小及染料包封对体外培养的管道动脉功能的影响。将罗丹明B异硫氰酸酯（RBITC）染料分子包封在二氧化硅壳中以产生小于100 nm尺寸的纳米颗粒（二氧化硅RBITC纳米颗粒）。研究了它们对内皮依赖性（乙酰胆碱; 0.01-200 µM）和非依赖性（硝普钠; 0.001-10 µM）扩张反应的影响。结果当与1.96 × 10（12）纳米颗粒/ml孵育时，30和70 nm SiNPs和二氧化硅RBITC纳米颗粒均显著降低内皮依赖性但非非依赖性血管舒张。衰减的程度与纳米颗粒的表面积有关，而不是尺寸，并受染料封装的影响。此外，由于二氧化硅RBITC纳米颗粒，但不是SiNPs，衰减的扩张，可以部分恢复使用超氧化物歧化酶。结论SiNPs和RBITC纳米粒的血管扩张衰减机制不同，这对进一步制备生物相容性纳米粒用于影像诊断具有重要意义。
AIM To determine the influence of silica nanoparticle (SiNP) number, size and dye encapsulation on conduit arterial function, in vitro. MATERIALS & METHODS Rhodamine B isothiocyanate (RBITC) dye molecules were encapsulated in a silica shell to produce nanoparticles (silica RBITC nanoparticles) smaller than 100 nm size. Their effects on endothelial-dependent (acetylcholine; 0.01-200 µM) and -independent (sodium nitroprusside; 0.001-10 µM) dilator responses were examined. RESULTS When incubated with 1.96 × 10(12) nanoparticles/ml, both 30 and 70 nm SiNPs and silica RBITC nanoparticles significantly reduced endothelium-dependent, but not -independent, vasodilation. The degree of attenuation was related to nanoparticle surface area, rather than size, and influenced by dye encapsulation. Furthermore, attenuated dilation due to silica RBITC nanoparticles, but not SiNPs, could be partially restored using superoxide dismutase. CONCLUSION Our results suggest that the mechanism of attenuated dilation is different for SiNPs and silica RBITC nanoparticles, which has implications for the future fabrication of biocompatible nanoparticles for imaging diagnostics.