Identification and discrimination of bacteria using Fourier transform infrared spectroscopy
Identification and discrimination of bacteria using Fourier transform infrared spectroscopy
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DOI：
10.1016/j.saa.2013.07.062
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发表时间：
2013-12-01
影响因子：
4.4
通讯作者：
Kulp, Thomas R.
中科院分区：
文献类型：
--
作者：
Maity, Jyoti Prakash;Kar, Sandeep;Kulp, Thomas R.
利用傅里叶红外光谱法在4000 ~ 600 cm（-1）波数范围内获得细菌光谱。分析了从油泥中分离的菌株OS1和OS2的FTIR光谱特征，并将其与16S-rRNA特征进行了匹配。从OSi （FJ226761）、参考菌株弯曲芽孢杆菌（ATCC 49095）、OS2 （FJ215874）和参考菌株嗜麦芽寡养单胞菌（ATCC 19861）中分别获得的特异光谱波段表明，051与ATCC 49095亲缘关系密切，而OS2则不同。这些条带可能代表特定细菌的蛋白质群和脂质。flexus的分离峰与OS1相似。S. maltophiia （ATCC 19861）和OS2在3272 cm（-1）处有相似的峰。酰胺带（I， II和III）显示OSi与B. flexus亲缘关系密切，但与OS2不同。在指纹区，1096 cm（-1）和1360 cm（-1）处出现了特异指纹。OS2和参考菌株嗜麦芽葡萄球菌（ATCC 19861）的差异。OS(1)和B. flexus ATCC 49095分别在1339 cm（-1）和1382 cm（-1）处发现了特异的指纹特征，从而可以区分这两种菌株。这种光谱特征来源于细胞的磷脂和RNA成分。主成分分析（PCA）表明，弹性芽孢杆菌（ATCC 49095）、嗜麦芽芽孢杆菌（ATCC 19861）、OS1和OS2在酰胺区和指纹区具有明显的样本聚类。(C) 2013 Elsevier B.V.版权所有
Bacterial spectra were obtained in the wavenumber range of 4000-600 cm(-1) using FTIR spectroscopy. FTIR spectral patterns were analyzed and matched with 16S-rRNA signatures of bacterial strains OS1 and OS2, isolated from oil sludge. Specific spectral bands obtained from OSi (FJ226761), reference strainBacillus flexus (ATCC 49095), OS2 (FJ215874) and reference strain Stenotrophomonas maltophilia (ATCC 19861) respectively, suggested that 051 and ATCC 49095 were closely related whereas OS2 was different. The bands probably represent groups of proteins and lipids of specific bacteria. Separate peaks found in B. flexus were similar to those of OS1. The S. maltophilia (ATCC 19861) and OS2 exhibited a similar peak at 3272 cm(-1). Amide bands (I, II and III) exhibited that OSi and B. flexus were closely related, but were different from OS2. In the finger-print region, peak at 1096 cm(-1) and 1360 cm(-1) exhibited the specific fingerprints. of OS2 and reference strain S. maltophilia (ATCC 19861), respectively. The specific fingerprint signature was found at 1339 cm(-1) for OS(1)and at 1382 cm(-1) for B. flexus ATCC 49095, allowing these two strains of B. flexus to be differentiated. This spectral signature originated from phospholipid and RNA components of the cell. Principle components analysis (PCA) of spectral regions exhibited with distinct sample clusters between Bacillus flexus (ATCC 49095), S. maltophilia (ATCC 19861), OS1 and OS2 in amide and fingerprint region. (C) 2013 Elsevier B.V. All rights reserved.