X-ray diffraction studies of whole rat heart during anoxic perfusion.
X-ray diffraction studies of whole rat heart during anoxic perfusion.
复制标题
缺氧灌注期间整个大鼠心脏的 X 射线衍射研究。
DOI：
10.1006/bbrc.1994.2064
复制
发表时间：
1994
影响因子：
3.1
通讯作者：
A. Stier
中科院分区：
文献类型：
--
作者：
A. Sowerby;J. Harries;G. Diakun;E. Towns;J. Bordas;A. Stier
记录了大鼠全心从常氧到缺氧过渡过程中心室区域的赤道x线反射。正常灌注时1.0和1.1赤道反射的强度比（I 1.0/I 1.1）为2.96，缺氧灌注115分钟后降至0.37，与部分解剖的松弛和僵硬脊椎动物心肌的强度比（1.2）非常接近。在缺氧灌注期间，这些反射的峰值位置都增加了约5%，表明肌节纤维晶格的横向扩张。这些结果表明，缺氧过程导致大多数肌球蛋白交叉桥与细丝结合的状态。使用这种技术可以研究整个心脏结构的整体变化，并且由于心脏易于灌注，可以研究与整个心脏结构相关的生化和生理问题。这可能具有临床意义，特别是在器官保存和移植的研究方面。这被认为是第一次用这种方法研究完整的肌肉器官。
Equatorial x-ray reflections were recorded from the ventricular region of whole rat heart, during the transition from normoxia to anoxia. The intensity ratio of the 1.0 and 1.1 equatorial reflections (I 1.0/I 1.1) was 2.96 during normoxic perfusion, decreasing to 0.37 after 115 minutes of anoxic perfusion and closely parallel those reported between partially dissected relaxed and rigor vertebrate heart muscle (1.2). The peak positions of these reflections both increased by ca. 5% during anoxic perfusion indicative of a lateral expansion in the sarcomere filament lattice. These results indicate that the process of anoxia leads to the condition of rigor in which the majority of the myosin cross-bridges bind to the thin filament. Using this technique global changes in whole heart structure can be studied, and due to the ease of perfusion of the heart, biochemical and physiological problems may be investigated in relation to the structure of the heart as a whole. This may be of clinical interest, particularly in terms of investigations into organ preservation and transplantation. This is believed to be the first occasion where intact muscular organs have been studied in this way.