ZNF207, a ubiquitously expressed zinc finger gene on chromosome 6p21.3.
ZNF207, a ubiquitously expressed zinc finger gene on chromosome 6p21.3.
复制标题
ZNF207，染色体 6p21.3 上普遍表达的锌指基因。
DOI：
10.1006/geno.1998.5442
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Horwitz,LD
中科院分区：
文献类型：
--
作者：
Pahl,PM;Hodges,YK;Meltesen,L;Perryman,MB;Horwitz,KB;Horwitz,LD
We have used differential display (5, 6) to examine transcripts present in human vascular smooth muscle cells (VSMC). One message, ZNF207, was cloned and sequenced. DNA sequencing was performed by the Biotechnology Resource Center of Cornell University using an ABI automated sequencer. A BLASTN search (1) with this sequence identified only one homologous sequence, X74802, which is a partial cDNA sequence of unknown function. A BLASTN search of the dbEST database indicated that the ZNF207 sequence has been identified numerous times as an expressed sequence in libraries made from a variety of tissues, including brain, breast, CNS, colon, foreskin, germ cell, heart, liver, lung, muscle, prostate, tonsil, uterus, and whole embryo. The gene is represented by 79 ESTs in the NCBI Unigene database (8) entry Hs. 62112. The 2347-bp cDNA sequence (Fig. 1) was determined by completely sequencing several overlapping clones that were obtained through the IMAGE Consortium (4). The cDNA sequence includes 202 bp of 5! untranslated region with a 64-bp AG repeat, a 1437-bp open reading frame, and a 708-bp 3! untranslated region with a poly (A) tail. IMAGE clone 39201 has a 54-bp in-frame deletion, which may have resulted from alternative splicing of the message. A BLASTN search using the complete ZNF207 cDNA sequence as query found no homologous human sequences, but identified a genomic Caenorhabditis elegans sequence (Z73102) that is 75% identical to ZNF207. PCR of the ZNF207 cDNA using primers E08A (5!-GCCCTCACCAAGCTAGGTTGGGG-3!) and E08B (5!-GCCACCACAGCCAGGCATCCC-3!) was performed for 35 cycles (94 C for 30 s, 65 C for 30 s, 72 C for 30 s), generating a 640-bp product. This amplimer was labeled with [32P] dCTP using the Prime-It II system (Stratagene). The labeled product was used as a probe with a human RNA Master Blot (Clontech) according to the manufacturer’s specifications. Hybridization was detected with RNA from all of the 43 adult and 7 fetal human tissues represented, but not with the negative control RNAs (not shown). By reverse transcription PCR analysis it was determined that theZNF207 transcript is present in both quiescent and actively growing VSMC in culture and in cultured breast cancer cells. PCR with the E08A and E08B primers using genomic human DNA as template gave a 743-bp product. This amplimer was sequenced and found to be identical to the ZNF207 cDNA amplimer with an additional 143-bp insertion at base 1477. A genomic clone of ZNF207 was obtained by screening a human P1 library (7) by PCR using the E08A and E08B primers. The P1 clone, P85G7, was labeled and used as a probe in FISH (Fig. 1C). By FISH analysis, the ZNF207 gene is located on chromosome 6p21. 3. The predicted protein encoded by ZNF207 has a molecular mass of 50.7 kDa and is composed of nearly 20% proline residues (Fig. 1B). The protein sequence has a potential nuclear localization signal at the amino terminus and contains two C2–H2 zinc finger motifs (2, 3). A BLASTP search (1) using the ZNF207 protein sequence as query was performed. The only sequence identified as having extensive homology was a predicted C. elegans protein. Minor homologies to zinc finger proteins and proteins rich in proline were detected. Because of the zinc finger motifs, it is expected that ZNF207 encodes a DNA-binding protein and potentially a transcription factor. Preliminary results indicate that ZNF207 can regulate transcription of a ß-Gal reporter gene in HeLa cells. We have described a novel gene, ZNF207, which maps to chromosome 6p21. 3 and encodes a …
