Transcriptomics and metabolomics of dietary leucine excess.
Transcriptomics and metabolomics of dietary leucine excess.
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DOI：
10.1093/jn/135.6.1571s
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发表时间：
2005-06
期刊：
影响因子：
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通讯作者：
Kaori Matsuzaki;H. Kato;R. Sakai;Sakino Toue;M. Amao;Takeshi Kimura
中科院分区：
文献类型：
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作者：
Kaori Matsuzaki;H. Kato;R. Sakai;Sakino Toue;M. Amao;Takeshi Kimura
研究了用基础饮食或添加 1.5%、5%、10%、15% 和 30% 亮氨酸的饮食喂养 2 周的大鼠血浆代谢物以及生理和毒理学变量的变化。在同一实验中，使用 DNA 微阵列研究了喂食基础饮食或添加 5% 和 15% 亮氨酸的饮食的大鼠肝脏中基因表达的变化。代谢物与生理和毒理学变量的多变量相关性的聚类分析表明，与过量氮相关的变量与亮氨酸和α-酮异己酸聚集在一起。基因表达数据虽然是初步的，但表明亮氨酸分解代谢途径的酶的表达几乎没有变化，但与氮代谢和亮氨酸分解代谢下游的其他途径相关的酶发生了变化。这些数据似乎与过量的亮氨酸通过氮代谢超载发挥作用一致，并且尿素或α-酮异己酸可能是充足摄入量上限的早期标志。
Changes were investigated in plasma metabolites and physiological and toxicological variables in rats fed for 2 wk on a basal diet or diets with 1.5, 5, 10, 15, and 30% added leucine. In the same experiment, the changes in gene expression in livers of rats fed the basal diet or diets with 5% and 15% added leucine were investigated using DNA microarrays. Cluster analysis of multivariate correlations of metabolites and physiological and toxicological variables indicated that the variables associated with excess nitrogen clustered together with leucine and alpha-ketoisocaproate. The gene expression data, although preliminary, indicated that there was little change in the expression of enzymes of the catabolic pathways for leucine but that there were changes in enzymes associated with nitrogen metabolism and other pathways downstream of leucine catabolism. The data seem consistent with excess leucine exerting its effects through the overloading of nitrogen metabolism and that urea or alpha-ketoisocaproate could be an early marker for the upper limit of adequate intake.