Vector competence of Anopheles and Culex mosquitoes for Zika virus.
Vector competence of Anopheles and Culex mosquitoes for Zika virus.
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作者：
Dodson BL;Rasgon JL
寨卡病毒是一种新出现的蚊媒黄病毒，最近在新世界引起了大规模爆发，导致严重疾病病理急剧增加，包括格林-巴利综合征、新生儿小头畸形和婴儿脑损伤。尽管伊蚊被认为是导致感染的主要蚊子种类，但该病毒已从包括库蚊和按蚊在内的数十种蚊子中分离出来，而且我们对哪些蚊子种类是病媒控制的目标缺乏透彻的了解。我们将冈比亚按蚊、史氏按蚊和致倦库蚊暴露于添加了两种寨卡病毒株的血粉中。吸血后 5 天、7 天和 14 天收集蚊子的身体、腿和唾液，并通过噬菌斑测定检测传染性病毒。无论滴度、病毒株或时间点如何，冈比亚按蚊、史氏按蚊和致倦库蚊对寨卡病毒感染都具有抵抗力。我们得出的结论是，冈比亚按蚊、斯蒂芬按蚊和致倦库蚊可能不会对寨卡病毒向人类的传播产生重大影响。然而，未来的研究应继续探索其他新型潜在载体传播病毒的可能性。
Zika virus is a newly emergent mosquito-borne flavivirus that has caused recent large outbreaks in the new world, leading to dramatic increases in serious disease pathology including Guillain-Barre syndrome, newborn microcephaly, and infant brain damage. Although Aedes mosquitoes are thought to be the primary mosquito species driving infection, the virus has been isolated from dozens of mosquito species, including Culex and Anopheles species, and we lack a thorough understanding of which mosquito species to target for vector control. We exposed Anopheles gambiae, Anopheles stephensi, and Culex quinquefasciatus mosquitoes to blood meals supplemented with two Zika virus strains. Mosquito bodies, legs, and saliva were collected five, seven, and 14 days post blood meal and tested for infectious virus by plaque assay. Regardless of titer, virus strain, or timepoint, Anopheles gambiae, Anopheles stephensi, and Culex quinquefasciatus mosquitoes were refractory to Zika virus infection. We conclude that Anopheles gambiae, Anopheles stephensi, and Culex quinquefasciatus mosquitoes likely do not contribute significantly to Zika virus transmission to humans. However, future studies should continue to explore the potential for other novel potential vectors to transmit the virus.