PHAGOCYTOSIS OF VIRULENT AND AVIRULENT LEPTOSPIRES BY GUINEA-PIG AND HUMAN POLYMORPHONUCLEAR LEUKOCYTES INVITRO
PHAGOCYTOSIS OF VIRULENT AND AVIRULENT LEPTOSPIRES BY GUINEA-PIG AND HUMAN POLYMORPHONUCLEAR LEUKOCYTES INVITRO
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DOI：
10.3109/00313028409068531
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发表时间：
1984-01-01
期刊：
影响因子：
4.5
通讯作者：
FAINE, S
中科院分区：
文献类型：
--
作者：
MCGRATH, H;ADLER, B;FAINE, S
本文用化学发光法和电镜观察了豚鼠和人中性粒细胞（PMN）对问号钩端螺旋体强毒株和弱毒株的吞噬作用。当豚鼠中性粒细胞与强毒钩端螺旋体孵育时，在存在但不存在特异性免疫血清的情况下，观察到显着的CL反应。添加豚鼠补体可显着增强这种反应。用电镜观察豚鼠中性粒细胞内的钩端螺旋体，证实了吞噬作用。豚鼠中性粒细胞对无毒力钩端螺旋体的吞噬作用和人中性粒细胞对毒力和无毒力钩端螺旋体的吞噬作用均需要特异性免疫血清和补体的存在。因此，在没有免疫血清的情况下，钩端螺旋体抵抗中性粒细胞吞噬作用的能力似乎不是毒力的主要决定因素。
Chemiluminescence (CL) and electron microscopy were used to study the phagocytosis of both virulent and avirulent strains of Leptospira interrogans serovar copenhageni by guinea pig and human polymorphonuclear leukocytes (PMN). A significant CL response was observed when guinea pig PMN were incubated with virulent leptospires in the presence but not in the absence of specific immune serum. This response was markedly enhanced by the addition of guinea pig complement. Phagocytosis was confirmed by the observation of intracellular leptospires in guinea pig PMN by EM. The phagocytosis of avirulent leptospires by guinea pig PMN and of both virulent and avirulent leptospires by human PMN required the presence of both specific immune serum and complement. Thus, the ability of leptospires to resist phagocytosis by PMN in the absence of immune serum does not appear to be a major determinant of virulence.