An unbiased metric of antiproliferative drug effect in vitro.
An unbiased metric of antiproliferative drug effect in vitro.
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DOI：
10.1038/nmeth.3852
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发表时间：
2016-06
期刊：
影响因子：
48
通讯作者：
Tyson DR
中科院分区：
文献类型：
--
作者：
Harris LA;Frick PL;Garbett SP;Hardeman KN;Paudel BB;Lopez CF;Quaranta V;Tyson DR
体外细胞增殖试验广泛应用于药理学、分子生物学和药物发现等领域。通过理论建模和实验，我们发现当前的抗增殖药物效应指标存在时间依赖的偏差，导致对药物效力和疗效等参数的不准确评估。我们提出了药物诱导增殖率(DIP)，即细胞群体倍增随时间变化的曲线的斜率，作为一种替代的、与时间无关的指标。
In vitro cell proliferation assays are widely used in pharmacology, molecular biology, and drug discovery. Using theoretical modeling and experimentation, we show that current antiproliferative drug effect metrics suffer from time-dependent bias, leading to inaccurate assessments of parameters such as drug potency and efficacy. We propose the drug-induced proliferation (DIP) rate, the slope of the line on a plot of cell population doublings versus time, as an alternative, time-independent metric.