Mouse urogenital development: a practical approach
Mouse urogenital development: a practical approach
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DOI：
10.1046/j.1432-0436.2003.7107004.x
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发表时间：
2003-09-01
期刊：
影响因子：
2.9
通讯作者：
Carroll, P
中科院分区：
文献类型：
--
作者：
Staack, A;Donjacour, AA;Carroll, P
需要详细了解胚胎小鼠泌尿生殖道的发育解剖学，以识别转基因和基因敲除小鼠的突变泌尿生殖表型。因此，本文的目的是审查小鼠胚胎泌尿生殖系统的发育，并给出一个说明性的方法协议，解剖泌尿生殖器官的雏形，在12-13天的妊娠（E12-13），分离泌尿生殖嵴和E16分离精囊，苗勒管，Wolffian管，和前列腺的雏形，泌尿生殖窦（UGS）。可以培养UGS，并且在睾酮存在下，在体外形成前列腺芽。由于间充质-上皮相互作用在泌尿生殖发育中的重要性，本文还描述了从胚胎泌尿生殖窦分离上皮和间充质的方法。尿生殖窦间充质（UGM）和尿生殖窦上皮（UGE）可用于构建组织重组体，其可在体外生长或在体内移植，用于研究前列腺发育中的上皮-间充质相互作用。
A detailed knowledge of the developmental anatomy of the embryonic mouse urogenital tract is required to recognize mutant urogenital phenotypes in transgenic and knock-out mice. Accordingly, the purpose of this article is to review urogenital development in the mouse embryo and to give an illustrated methodological protocol for the dissection of urogenital organ rudiments at 12-13 days of gestation (E12-13) to isolate the urogenital ridge and at E16 to isolate the seminal vesicle, Mullerian duct, Wolffian duct, and prostatic rudiment, the urogenital sinus (UGS). The UGS can be cultured and, in the presence of testosterone, prostatic buds form in vitro. Because of the importance of mesenchymal-epithelial interactions in urogenital development, methods for the isolation of epithelium and mesenchyme from the embryonic urogenital sinus are also described. Urogenital sinus mesenchyme (UGM) and urogenital sinus epithelium (UGE) can be used to construct tissue recombinants that can either be grown in vitro or grafted in vivo for the study of epithelial-mesenchymal interactions in prostatic development.