Transcription modulation by a rat nuclear scaffold protein, P130, and a rat highly repetitive DNA component or various types of animal and plant matrix or scaffold attachment regions
Transcription modulation by a rat nuclear scaffold protein, P130, and a rat highly repetitive DNA component or various types of animal and plant matrix or scaffold attachment regions
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DOI：
10.1006/bbrc.2000.3938
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发表时间：
2000-12-09
影响因子：
3.1
通讯作者：
Hiraga, K
中科院分区：
文献类型：
--
作者：
Hibino, Y;Ohzeki, H;Hiraga, K
研究了高重复DNA成分XmnI片段与动植物基质或支架附着区（MAR/SAR）与核支架相互作用的相似性。由于XmnI片段在体外结合了130 kDa的支架蛋白（P130），因此各种类型的MAR/SAR片段可以结合130和123 kDa的支架蛋白。在将重组质粒瞬时转染到不同类型的受体细胞后，原生XmnI和MAR/SAR片段明显增强了SV40启动子介导的荧光素酶基因转录。相比之下，在独特的HindIII位点的胞嘧啶碱基甲基化的XmnI片段，以及缺乏MAR/ SAR碱基解配对特征的合成变异DNA，既不能结合P130，也不能增强这种转录。这两种类型的基因组区域似乎具有与核支架相互作用的相似特性，从而可以调节适当定位的启动子的活性。(C) 2000年学术出版社。
The XmnI fragment, a highly repetitive DNA component, and animal and plant matrix or scaffold attachment region (MAR/SAR) were examined for similarity in interaction with nuclear scaffold. As the XmnI fragment bound a 130 kDa scaffold protein (P130) in vitro, various types of MAR/SAR fragments could bind 130 and 123 kDa scaffold proteins. The native XmnI and MAR/SAR fragments clearly augmented SV40 promoter-mediated luciferase gene transcription following transient transfection of recombinant plasmids into various types of recipient cells. In contrast, the XmnI fragment methylated at the cytosine base of the unique HindIII site, and a synthetic variant DNA deficient in base unpairing characteristic of MAR/ SAR, could neither bind P130 nor augment this transcription. These two types of genomic regions appeared to have similar properties of interaction with nuclear scaffold, by which the activity of appropriately positioned promoter can be modulated. (C) 2000 Academic Press.