Engineering hybrid exosomes by membrane fusion with liposomes.
Engineering hybrid exosomes by membrane fusion with liposomes.
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DOI：
10.1038/srep21933
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发表时间：
2016-02-25
影响因子：
4.6
通讯作者：
Akiyoshi K
中科院分区：
文献类型：
--
作者：
Sato YT;Umezaki K;Sawada S;Mukai SA;Sasaki Y;Harada N;Shiku H;Akiyoshi K
外泌体是一种有价值的生物材料，用于开发新型纳米载体作为功能先进的药物输送系统。为了控制和修饰外泌体纳米载体的性能，我们利用冷冻-解冻方法将外泌体膜与脂质体融合，制备了杂交外泌体。从基因修饰细胞中分离的特定膜蛋白包埋的外泌体与各种脂质体融合，证实了膜工程方法可以与基因修饰技术相结合。使用杂交外泌体进行的细胞摄取研究表明，发育的外泌体和细胞之间的相互作用可以通过改变脂质组成或外源脂质的性质来修饰。这些结果表明，本文报道的膜工程方法为开发合理设计的外泌体作为混合纳米载体用于先进的药物输送系统提供了一种新的策略。
Exosomes are a valuable biomaterial for the development of novel nanocarriers as functionally advanced drug delivery systems. To control and modify the performance of exosomal nanocarriers, we developed hybrid exosomes by fusing their membranes with liposomes using the freeze–thaw method. Exosomes embedded with a specific membrane protein isolated from genetically modified cells were fused with various liposomes, confirming that membrane engineering methods can be combined with genetic modification techniques. Cellular uptake studies performed using the hybrid exosomes revealed that the interactions between the developed exosomes and cells could be modified by changing the lipid composition or the properties of the exogenous lipids. These results suggest that the membrane-engineering approach reported here offers a new strategy for developing rationally designed exosomes as hybrid nanocarriers for use in advanced drug delivery systems.