Fructan synthesis, accumulation and polymer traits. II. Fructan pools in populations of perennial ryegrass (Lolium perenne L.) with variation for water-soluble carbohydrate and candidate genes were not correlated with biosynthetic activity and demonstrated constraints to polymer chain extension.
Fructan synthesis, accumulation and polymer traits. II. Fructan pools in populations of perennial ryegrass (Lolium perenne L.) with variation for water-soluble carbohydrate and candidate genes were not correlated with biosynthetic activity and demonstrated constraints to polymer chain extension.
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DOI：
10.3389/fpls.2015.00864
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发表时间：
2015
影响因子：
5.6
通讯作者：
Turner LB
中科院分区：
文献类型：
--
作者：
Gallagher JA;Cairns AJ;Thomas D;Timms-Taravella E;Skøt K;Charlton A;Williams P;Turner LB
黑麦草和羊茅在Festulolium亚线基因渗入家族中在果聚糖合成、代谢和聚合物大小性状上存在差异。多年生黑麦草的水溶性碳水化合物和果聚糖含量有很大的差异。然而，关于这个物种的果聚糖聚合物池，特别是关于它的组成和调控，还有很多有待发现。假设聚合物特性可能存在类似的相当大的变化，为生物精制应用提供有用的聚合物。研究了不同多年生黑麦草材料对果聚糖含量、果聚糖合成和聚合物大小性状的季节性影响，这些材料包括多年生黑麦草F2作图家族和三轮表型选择产生的群体的对比植株。研究了候选基因与拷贝数变异的关系。在这些条件下，几乎没有证据表明这种不同种质的果聚糖代谢有任何变化，这导致了高水溶性碳水化合物浓度下叶组织中存在的果聚糖聚合物补体的实质性差异。在果聚糖积累过程中，果聚糖合成的重要性尚不清楚，因为在生长季节，完整植株的果聚糖含量和聚合物特征并不能反映其从头合成的能力。然而，在有利于高汇/低源需求的环境条件下，果聚糖的保留可能是高糖性状的重要组成部分，并讨论了分解和周转的作用。
Differences have been shown between ryegrass and fescue within the Festulolium subline introgression family for fructan synthesis, metabolism, and polymer-size traits. It is well-established that there is considerable variation for water-soluble carbohydrate and fructan content within perennial ryegrass. However there is much still to be discovered about the fructan polymer pool in this species, especially in regard to its composition and regulation. It is postulated that similar considerable variation for polymer traits may exist, providing useful polymers for biorefining applications. Seasonal effects on fructan content together with fructan synthesis and polymer-size traits have been examined in diverse perennial ryegrass material comprising contrasting plants from a perennial ryegrass F2 mapping family and from populations produced by three rounds of phenotypic selection. Relationships with copy number variation in candidate genes have been investigated. There was little evidence of any variation in fructan metabolism across this diverse germplasm under these conditions that resulted in substantial differences in the complement of fructan polymers present in leaf tissue at high water-soluble carbohydrate concentrations. The importance of fructan synthesis during fructan accumulation was unclear as fructan content and polymer characteristics in intact plants during the growing season did not reflect the capacity for de novo synthesis. However, the retention of fructan in environmental conditions favoring high sink/low source demand may be an important component of the high sugar trait and the roles of breakdown and turnover are discussed.