Metabolomic prediction of pregnancy viability in superovulated cattle embryos and recipients with fourier transform infrared spectroscopy.
Metabolomic prediction of pregnancy viability in superovulated cattle embryos and recipients with fourier transform infrared spectroscopy.
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利用傅里叶变换红外光谱对超排牛胚胎和受体妊娠活力进行代谢组学预测。
DOI：
10.1155/2014/608579
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发表时间：
2014
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作者：
Muñoz,Marta;Uyar,Asli;Correia,Eva;Ponsart,Claire;Guyader-Joly,Catherine;Martínez-Bello,Daniel;Marquant-LeGuienne,Brigitte;Fernandez-Gonzalez,Alfonso;Díez,Carmen;Caamaño,JoseNestor;Trigal,Beatriz;Humblot,Patrice;Carrocera,Susana;
我们使用傅立叶变换红外光谱（FTIR）代谢组学分析胚胎培养基（CM）和受体血浆，以确定预测妊娠结局的光谱模型。从两个国家的超排卵奶牛中收集第6天的胚胎，在含BSA的合成输卵管液培养基中单独培养24 h，然后进行胚胎移植。使用FTIR对废CM、空白对照和血浆样本（第0天和第7天）进行评估。对所得光谱进行了分析。对分类器的鉴别能力进行准确性、敏感性（妊娠）、特异性（非妊娠）和ROC曲线下面积（AUC）的评估。考虑的终点是怀孕和分娩第60天。在单个实验室中，CM妊娠第60天的AUC较高（法国AUC = 0.751±0.039，西班牙AUC = 0.718±0.024），而累积数据使AUC降低（AUC = 0.604±0.029）。出生时CM的预测低于妊娠第60天。出生时血浆预测的累积结果优于个体结果（第0天：法国AUC = 0.690±0.044；西班牙AUC < 0.55；累积AUC = 0.747±0.032）。血浆通常比CM更能预测妊娠和分娩。这些初步结果表明，FTIR代谢组学可以识别具有更高妊娠能力的胚胎和受体，这可能有助于提高基于ET的选择方案的效率。
We analyzed embryo culture medium (CM) and recipient blood plasma using Fourier transform infrared spectroscopy (FTIR) metabolomics to identify spectral models predictive of pregnancy outcome. Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured in synthetic oviduct fluid medium with BSA for 24 h before embryo transfer. Spent CM, blank controls, and plasma samples (Day 0 and Day 7) were evaluated using FTIR. The spectra obtained were analyzed. The discrimination capability of the classifiers was assessed for accuracy, sensitivity (pregnancy), specificity (nonpregnancy), and area under the ROC curve (AUC). Endpoints considered were Day 60 pregnancy and birth. High AUC was obtained for Day 60 pregnancy in CM within individual laboratories (France AUC = 0.751 ± 0.039, Spain AUC = 0.718 ± 0.024), while cumulative data decreased the AUC (AUC = 0.604 ± 0.029). Predictions for CM at birth were lower than Day 60 pregnancy. Predictions with plasma at birth improved cumulative over individual results (Day 0: France AUC = 0.690 ± 0.044; Spain AUC < 0.55; cumulative AUC = 0.747 ± 0.032). Plasma generally predicted pregnancy and birth better than CM. These first results show that FTIR metabolomics could allow the identification of embryos and recipients with improved pregnancy viability, which may contribute to increasing the efficiency of selection schemes based on ET.