Self-assembling nanoprobes that display off/on 19F nuclear magnetic resonance signals for protein detection and imaging
Self-assembling nanoprobes that display off/on 19F nuclear magnetic resonance signals for protein detection and imaging
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DOI：
10.1038/nchem.365
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发表时间：
2009-10-01
期刊：
影响因子：
21.8
通讯作者：
Hamachi, Itaru
中科院分区：
文献类型：
--
作者：
Takaoka, Yousuke;Sakamoto, Takashi;Hamachi, Itaru
磁共振成像（MRI）是一种最有前途的技术，用于生物标志物和生物相关物种的非侵入性可视化，在体内和体外。虽然使用顺磁造影剂（例如Gd 3+络合物和氧化铁）的H-1 MRI被广泛使用，但是由于生物样品中大量质子分布引起的大背景信号，其经常遭受低对比度。在这里，我们报告了使用超分子有机纳米粒子来检测特定的蛋白质的F-19为基础的MRI在一个关闭/打开模式。在NMR光谱学中，这些设计的探针在聚集时是沉默的，但在靶蛋白存在的情况下，它们分解产生尖锐的信号。这种“开启”反应使我们能够通过F-19 MRI清楚地观察活细胞内的蛋白质，并构建细胞内抑制剂测定。这种用于开启F-19信号的纳米探针的分解驱动是前所未有的，并且可能扩展F-19 MRI用于特定蛋白质成像的用途。
Magnetic resonance imaging (MRI) is one of the most promising techniques for the non-invasive visualization of biomarkers and biologically relevant species, both in vivo and ex vivo. Although H-1 MRI with paramagnetic contrast agents, such as Gd3+ complexes and iron oxide, is widely used, it often suffers from low contrast because of the large background signals caused by the abundant distribution of protons in biological samples. Here we report the use of supramolecular organic nanoparticles to detect specific proteins by F-19-based MRI in an off/on mode. In NMR spectroscopy these designed probes are silent when aggregated, but in the presence of a target protein they disassemble to produce a sharp signal. This 'turn-on' response allowed us to visualize clearly the protein within live cells by F-19 MRI and construct an in-cell inhibitor assay. This recognition-driven disassembly of nanoprobes for a turn-on F-19 signal is unprecedented and may extend the use of F-19 MRI for specific protein imaging.