Antifungal activity of secondary metabolites of Pseudomonas fluorescens isolates as a biocontrol agent of chocolate spot disease (Botrytis fabae) of faba bean in Ethiopia
Antifungal activity of secondary metabolites of Pseudomonas fluorescens isolates as a biocontrol agent of chocolate spot disease (Botrytis fabae) of faba bean in Ethiopia
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DOI：
10.5897/ajmr2013.5899
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发表时间：
2013-11
影响因子：
--
通讯作者：
F. Alemu;T. Alemu
中科院分区：
文献类型：
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作者：
F. Alemu;T. Alemu
荧光假单胞菌分离株具有多种有前景的次级代谢产物抗真菌活性特性，使其成为生物防治剂。在本研究中，从蚕豆作物根际土壤中分离出 12 个荧光假单胞菌，评估其对蚕豆巧克力斑病（Botrytis fabae）的拮抗活性。荧光假单胞菌 10 (Pf 10) (88.1%) 对豆芽孢杆菌表现出高拮抗活性。由于其抗真菌代谢物，荧光假单胞菌的所有分离株均成功用于控制蚕豆巧克力斑病。用等体积的乙酸乙酯、己烷和甲醇从所有荧光假单胞菌分离物中提取抗真菌化合物。用乙酸乙酯、己烷和甲醇从荧光假单胞菌 3 (Pf 3)、荧光假单胞菌 8 (Pf 8) 和荧光假单胞菌 3 (Pf 3) 分离株中提取的抗真菌化合物在 0.1% 浓度下分别完全抑制病原体的菌丝生长。含有荧光假单胞菌 9 (Pf 9) 和荧光假单胞菌 10 (Pf 10) 分离株的生物底漆蚕豆种子在体内（盆栽培养）中针对蚕豆芽胞杆菌进行了评估，表明对病原体有抑制作用，并且还显示出增强作物免疫系统的诱导特性。因此，可以得出结论，使用分离株荧光假单胞菌9（Pf 9）和荧光假单胞菌10（Pf 10）可以抑制蚕豆菌丝体的生长，减少蚕豆的发病率、严重程度和侵染过程，同时提高蚕豆的植株生长性能和产量。这些分离株可用作对抗蚕豆的潜在生物防治剂，也可用作蚕豆生产的生物肥料。关键词：抗真菌，生物防治，蚕豆灰霉病，蚕豆，荧光假单胞菌。
Pseudomonas fluorescens isolates possess a variety of promising properties of antifungal activity of secondary metabolites which make it as a biocontrol agent. In the present study, 12 isolates of P. fluorescens were isolated from rhizospheric soil of faba bean crop evaluated for their antagonistic activity against chocolate spot disease (Botrytis fabae) of faba bean. P. fluorescens 10 (Pf 10) (88.1%) showed high antagonistic activity against B. fabae. All isolate of P. fluorescens were successfully employed in controlling chocolate spot disease of faba bean due to their antifungal metabolites. The antifungal compounds were extracted from all P. fluorescens isolates with equal volume of ethyl acetate, hexane and methanol. The antifungal compounds extracted with ethyl acetate, hexane and methanol from P. fluorescens 3 (Pf 3), P. fluorescens 8 (Pf 8), and P. fluorescens 3 (Pf 3), isolates at 0.1% concentration completely inhibited the mycelial growth of the pathogen respectively. Bio-primed faba bean seeds with isolates of P. fluorescens 9 (Pf 9) and P. fluorescens 10 (Pf 10) evaluated against B. fabae in vivo (pot culture) indicated the inhibitory effects to the pathogen and also showed the inducing properties to enhance the immune system of crop. Therefore, it can be concluded that the use of P. fluorescens 9 (Pf 9) and P. fluorescens 10 (Pf 10) of isolates could inhibit the mycelial growth and reduced the disease incidence, severity and infection processes of B. fabae and simultaneously increase the plant growth performance and yield of faba bean. These isolates can be used as potential biocontrol agents against B. fabae and also used as biofertilizers for the production of faba bean. Key words: Antifungal, Biocontrol, Botrytis fabae, Faba bean, Pseudomonas fluorescens.