Collagen VI Is a Subepithelial Adhesive Target for Human Respiratory Tract Pathogens
Collagen VI Is a Subepithelial Adhesive Target for Human Respiratory Tract Pathogens
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DOI：
10.1159/000232587
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发表时间：
2010-01-01
影响因子：
5.3
通讯作者：
Morgelin, Matthias
中科院分区：
文献类型：
--
作者：
Bober, Marta;Enochsson, Charlotte;Morgelin, Matthias
细菌对特定宿主组织结构的接触可以作为一种手段，将病原体定位于特定的生态位，建立持续感染并诱导入侵。在这种情况下，初级黏附通常是第一个关键的定植步骤，使病原体能够抵抗宿主的机械清除机制。因此，细菌进化出粘附素，具有调节微生物和宿主之间相互作用的能力。在此，我们将VI胶原蛋白描述为化脓性链球菌和肺炎链球菌黏附的新靶点。在上呼吸道和下呼吸道，该胶原分布于上皮基底膜下的固有层。光镜和电子显微镜结合免疫检测和体外结合试验表明，两种病原体对VI型胶原均有较强的亲和力。对于化脓性链球菌，这种相互作用是由M1蛋白介导的。目前的数据为VI型胶原在呼吸道感染期间宿主-病原体相互作用中先前未知的作用提供了证据。版权所有(C)2009 S.Karger AG，巴塞尔
Bacterial engagement of specific host tissue structures can be a means of targeting a pathogen to a particular niche, establishing persistent infections and inducing invasion. In this context, primary adhesion is often the first crucial colonization step allowing pathogens to withstand the mechanical clearing mechanisms of the host. As a consequence, bacteria have evolved adhesins with the capacity to mediate interaction between microorganism and host. Here we describe Collagen VI as a novel target for adherence of Streptococcus pyogenes and Streptococcus pneumoniae. In upper and lower airways this Collagen was distributed in the lamina propria underneath the epithelial basement membrane. Both pathogens exhibited strong affinity to Collagen VI as shown by light and electron microscopy in combination with immunodetection and in vitro binding assays. For S. pyogenes this interaction was mediated by M1 protein. The presented data provide evidence for a previously unrecognized role for Collagen VI in host-pathogen interplay during respiratory tract infection. Copyright (C) 2009 S. Karger AG, Basel