An iTRAQ-based quantitative analysis to elaborate the proteomic response of Nostoc sp. PCC 7120 under N2 fixing conditions.
An iTRAQ-based quantitative analysis to elaborate the proteomic response of Nostoc sp. PCC 7120 under N2 fixing conditions.
复制标题
基于 iTRAQ 的定量分析，详细阐述发菜的蛋白质组学反应。
DOI：
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发表时间：
2007
影响因子：
4.4
通讯作者：
P. Wright
中科院分区：
文献类型：
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作者：
Karin Stensjö;S. Ow;M. Barrios;P. Lindblad;P. Wright
发菜属Nostoc sp.PCC7120是一种固定丝状蓝藻的放氧光自养氮气。在氮饥饿的情况下，一系列的过程被启动，例如异形胞的分化，发生氮气固定的特定细胞。我们对发菜的蛋白质组进行了鉴定和定量。在固氮和非固氮条件下生长的PCC7120野生型菌株。为了评估全球蛋白质组对环境变化的响应，使用定量蛋白质组学工具iTRAQ对整个细胞消化进行了测量。通过这种方法，在2个生物复制实验中总共准确地鉴定了486个不同的蛋白质，其中226个鉴定包含2个或更多不同的多肽。代谢调控的结果将被讨论，以证明蛋白质组学是发展异晶蓝藻以用于未来生物制氢的重要工具。
Nostoc sp. PCC 7120 is an oxygen-evolving photoautotrophic N2 fixing filamentous cyanobacterium. Upon nitrogen starvation, a range of processes are initiated, such as differentiation of the heterocysts, specific cells where N2 fixation takes place. We have characterized and quantified the proteome of the Nostoc sp. PCC 7120 wild-type strain grown under N2 fixing and non-N2 fixing conditions. To assess global proteome changes in response to environmental changes, measurements were made using the quantitative proteomics tool, iTRAQ, on a whole cell digest. From this approach, a total of 486 different proteins was accurately identified across 2 biological replicate experiments, where 226 identifications contained 2 or more distinct peptides. Results of metabolic regulation will be discussed to demonstrate that proteomics represents an important tool for the development of heterocystous cyanobacteria for future biological H2 production.