The WRKY Transcription Factor GmWRKY12 Confers Drought and Salt Tolerance in Soybean
The WRKY Transcription Factor GmWRKY12 Confers Drought and Salt Tolerance in Soybean
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DOI：
10.3390/ijms19124087
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发表时间：
2018-12-01
影响因子：
5.6
通讯作者：
Zhang, Xiao-Hong
中科院分区：
文献类型：
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作者：
Shi, Wen-Yan;Du, Yong-Tao;Zhang, Xiao-Hong
wrkey是植物发育和逆境响应的重要调控因子。然而，对大豆中这一超家族的认识有限。在这项研究中，我们基于RNA-Seq和qRT-PCR对干旱和盐诱导基因GmWRKY12进行了鉴定。GmWRKY12全长714bp，编码237个氨基酸，隶属于WRKY II。GmWRKY12的启动子区包括ABER4、MYB、MYC、GT-1、W-box和DPBF顺式元件，可能参与ABA、干旱和盐胁迫响应。GmWRKY12在正常条件下在不同组织中表达量最低，而在干旱和盐处理下表达量较高。GmWRKY12作为核蛋白，对干旱、盐、ABA和水杨酸（SA）胁迫均有响应。通过转基因毛状根实验，我们进一步鉴定了GmWRKY12在非生物胁迫抗性中的作用。与对照（Williams 82）相比，GmWRKY12的过表达增强了转基因大豆幼苗在干旱和盐处理下的耐旱性和耐盐性，增加了脯氨酸（Pro）含量，降低了丙二醛（MDA）含量。这些结果为进一步了解GmWRKY12在大豆非生物胁迫响应中的功能提供了依据。
WRKYs are important regulators in plant development and stress responses. However, knowledge of this superfamily in soybean is limited. In this study, we characterized the drought- and salt-induced gene GmWRKY12 based on RNA-Seq and qRT-PCR. GmWRKY12, which is 714 bp in length, encoded 237 amino acids and grouped into WRKY II. The promoter region of GmWRKY12 included ABER4, MYB, MYC, GT-1, W-box and DPBF cis-elements, which possibly participate in abscisic acid (ABA), drought and salt stress responses. GmWRKY12 was minimally expressed in different tissues under normal conditions but highly expressed under drought and salt treatments. As a nucleus protein, GmWRKY12 was responsive to drought, salt, ABA and salicylic acid (SA) stresses. Using a transgenic hairy root assay, we further characterized the roles of GmWRKY12 in abiotic stress tolerance. Compared with control (Williams 82), overexpression of GmWRKY12 enhanced drought and salt tolerance, increased proline (Pro) content and decreased malondialdehyde (MDA) content under drought and salt treatment in transgenic soybean seedlings. These results may provide a basis to understand the functions of GmWRKY12 in abiotic stress responses in soybean.