Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary?
Preconditioning of Bioactive Glasses before Introduction to Static Cell Culture: What Is Really Necessary?
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DOI：
10.3390/mps3020038
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发表时间：
2020-06-01
影响因子：
2.4
通讯作者：
Westhauser, Fabian
中科院分区：
文献类型：
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作者：
Hohenbild, Frederike;Arango-Ospina, Marcela;Westhauser, Fabian
由于其高生物活性，在体外分析的生物活性玻璃（BG）可以是具有挑战性的，当涉及到保持生理pH值。为了提高BG的生物相容性，一个异质谱的预处理方法，如“钝化”的BG细胞培养基中孵育，使用，但从来没有直接比较。在本研究中，评价了长达72 h的钝化期对人骨髓来源的间充质基质细胞的pH碱化和活力的影响，以确定使用基于不同浓度的45 S5-BG组合物（以重量%计：45.0 SiO2，24.5 Na 2 O，24.5 CaO，6.0 P2 O 5）的颗粒的时间有效的钝化方案。pH碱化在钝化24 h后降低最多。与未钝化的45 S5-BG相比，在钝化至少24小时后，细胞活力随着钝化时间的增加而持续改善，并且所需的钝化时间随着BG浓度的增加而增加。在这种情况下，24小时的钝化期是提供生物相容性细胞培养环境的有效方法。总之，在将BG引入细胞培养物之前，应评估不同的钝化期，以满足相应的实验设置，例如，通过遵循本研究中使用的实验方案。
Due to their high bioreactivity, the in-vitro analysis of bioactive glasses (BGs) can be challenging when it comes to maintaining a physiological pH. To improve BG biocompatibility, a heterogenic spectrum of preconditioning approaches, such as "passivation" of the BGs by incubation in cell culture medium, are used but have never been directly compared. In this study, the effect of passivation periods of up to 72 h on pH alkalization and viability of human bone marrow-derived mesenchymal stromal cells was evaluated to determine a time-efficient passivation protocol using granules based on the 45S5-BG composition (in wt%: 45.0 SiO2, 24.5 Na2O, 24.5 CaO, 6.0 P2O5) in different concentrations. pH alkalization was most reduced after passivation of 24 h. Cell viability continuously improved with increasing passivation time being significantly higher after passivation of at least 24 h compared to non-passivated 45S5-BG and the necessary passivation time increased with increasing BG concentrations. In this setting, a passivation period of 24 h presented as an effective approach to provide a biocompatible cell culture setting. In conclusion, before introduction of BGs in cell culture, different passivation periods should be evaluated in order to meet the respective experimental settings, e.g., by following the experimental protocols used in this study.