Selective Rare Earth Recovery Employing Filamentous Viruses with Chemically Conjugated Peptides
Selective Rare Earth Recovery Employing Filamentous Viruses with Chemically Conjugated Peptides
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DOI：
10.1002/slct.201600542
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发表时间：
2016-07-16
期刊：
影响因子：
2.1
通讯作者：
Serizawa, Takeshi
中科院分区：
文献类型：
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作者：
Sawada, Toshiki;Asada, Masaya;Serizawa, Takeshi
从工业废料中选择性地回收稀土元素对循环利用过程和绿色经济至关重要。在这里，我们开发了一种基于丝状病毒（噬菌体）的钕离子（Nd3+）选择性回收系统，Nd3+通常用于由钕铁硼合金组成的普通稀土磁体。对Nd3+的亲和依赖性肽筛选产生的噬菌体克隆显示对Nd3+具有特定亲和性的肽。结合分析表明，该肽对Nd3+而非Fe3+具有特异性亲和力。结构分析表明，当该肽与Nd3+结合时，它经历了从随机线圈结构到β匝结构的结构转变，对Nd3+具有特异性亲和力。在含有Nd3+和过量Fe3+的混合溶液中，Nd3+吸附在肽修饰噬菌体上的量约为Fe3+的6倍，这表明具有Nd3+结合肽的适当修饰噬菌体可以作为Nd3+的选择性回收支架。
Selective recovery of rare earth elements from industrial waste is essential for recycling processes and a green economy. Here, we developed a filamentous virus (phage)-based selective recovery system for neodymium ions (Nd3+), which are generally used in common rare earth magnets composed of neodymiumiron- boron alloys. Affinity-dependent peptide screening against Nd3+ produced phage clones displaying peptides with a specific affinity for Nd3+. Binding analyses revealed that the peptide had a specific affinity for Nd3+ rather than Fe3+. Structural analyses of the peptide showed that when the peptide bound to Nd3+, it underwent a structural transition from random-coil to beta-turn structures, resulting in specific affinity for Nd3+. Approximately 6 times more Nd3+ adsorbed onto the peptide-modified phage than Fe3+ from a mixed solution containing Nd3+ and excess Fe3+, demonstrating that suitably modified phages with Nd3+-binding peptides can act as a selective recovery scaffold for Nd3+.