Tissue-specific distribution of donor mitochondrial DNA in cloned mice produced by somatic cell nuclear transfer
Tissue-specific distribution of donor mitochondrial DNA in cloned mice produced by somatic cell nuclear transfer
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DOI：
10.1002/gene.20029
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发表时间：
2004-06-01
期刊：
影响因子：
1.5
通讯作者：
Ogura, A
中科院分区：
文献类型：
--
作者：
Inoue, K;Ogonuki, N;Ogura, A
核移植家畜的线粒体DNA异质性研究结果差异很大。在这项研究中，我们克隆了遗传定义的小鼠和研究供体mtDNA遗传体细胞克隆。用受体卵母细胞或供体细胞特异性引物进行聚合酶链反应（PCR）分析，发现96%（24/25）的成体克隆的脑、肝、肾和尾组织中供体mtDNA与受体mtDNA共存。采用等位基因特异性定量PCR方法检测供体mtDNA在各组织中的比例，并进行方差分析，结果显示供体mtDNA在各组织中的分离模式存在显著性差异（P < 0.05），其中肝脏中供体mtDNA的比例最高。因此，供体mtDNA被克隆后代一致地遗传，而供体mtDNA分离不是中性的，这与先前关于mtDNA分离的组织特异性核控制的概念一致。(C)2004 Wiley-Liss，Inc.
Highly diverse results have been reported for mitochondrial DNA (mtDNA) hetero-plasmy in nuclear transferred farm animals. In this study, we cloned genetically defined mice and investigated donor mtDNA inheritance following somatic cell cloning. Polymerase chain reaction (PCR) analysis with primers that were specific for either the recipient oocytes or donor cells revealed that the donor mtDNA coexisted with the recipient mtDNA in the brain, liver, kidney, and tail tissues of 96% (24/25) of the adult clones. When the proportion of donor mtDNA in each tissue was measured by allele-specific quantitative PCR and subjected to ANOVA analysis, a tissue-specific mtDNA segregation pattern (P < 0.05) was observed, with the liver containing the highest proportion of donor mtDNA. Therefore, the donor mtDNA was inherited consistently by the cloned offspring, whereas donor mtDNA segregation was not neutral, which is in accordance with previous notions about tissue-specific nuclear control of mtDNA segregation. (C) 2004 Wiley-Liss, Inc.