Damage and recovery of the ovary in female zebrafish i.p.-injected with MC-LR
Damage and recovery of the ovary in female zebrafish i.p.-injected with MC-LR
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腹腔注射MC-LR对雌性斑马鱼卵巢的损伤和恢复
DOI：
10.1016/j.aquatox.2014.06.010
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发表时间：
2014-10-01
影响因子：
4.5
通讯作者：
Wu, Ning
中科院分区：
文献类型：
--
作者：
Hou, Jie;Li, Li;Wu, Ning
到目前为止，关于微囊藻毒素对生殖系统的毒性作用的体内研究还很有限，其诱导生殖毒性的潜在分子机制尚不清楚。在急性毒性实验中，将雌性斑马鱼(Danio Rerio)腹腔注射(Ip)。分别于注射后1、3、12、24、48和168h，分别以50和200mgMC-LR/kg体重(BW)处理卵巢，观察卵巢的组织病理变化、抗氧化酶活性和基因表达。在12HPI内，随着染毒时间和剂量的增加，斑马鱼卵巢的病理损伤程度和程度逐渐加重。同时，体内丙二醛(MDA)含量以及抗氧化酶过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPX)的活性和转录水平的升高，表明MC-LR对斑马鱼卵巢的结构和功能活性产生了不利的影响。氧化应激在MC-LR的生殖毒性中起重要作用。斑马鱼卵巢中谷胱甘肽含量的显著下降表明谷胱甘肽S转移酶通过谷胱甘肽转运酶对MC-LR解毒的重要性。组织结构和抗氧化指标的最终恢复表明，卵巢高效的抗氧化防御系统可能是斑马鱼对抗MC-LR的重要机制。虽然MC-LR的负面作用可以通过卵巢抗氧化系统来克服，但由于MC-LR的广泛存在，其潜在的生殖风险不容忽视。(C)2014爱思唯尔B.V.保留所有权利。
Up to now, in vivo studies on toxic effects of microcystins (MCs) on the reproductive system are limited and the underlying molecular mechanisms of MCs-induced reproductive toxicity remain to be elucidated. In an acute toxic experiment, female zebrafish (Danio rerio) were injected intraperitoneally (i.p.) at doses of 50 and 200 mu g MC-LR/kg body weight (BW) respectively, and histopathological lesions and antioxidant enzymatic activities and gene expression in the ovary were studied at 1, 3, 12, 24, 48 and 168 h post injection (hpi). Pathological lesions of zebrafish ovary progressed in severity and extent with the increasing exposure time and dose within 12 hpi. Concurrently, the increases in malondialdehyde (MDA) contents as well as the enzymatic activities and transcriptional levels of antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) showed the occurrence of oxidative stress, indicating that MC-LR induced adverse effects on the structure and functional activity of zebrafish ovary. Oxidative stress plays a significant role in the reproductive toxicity of MC-LR. The significant decrease of glutathione (GSH) content in zebrafish ovary suggested the importance of MC-LR detoxification by glutathione S-transferases (GST) via GSH. The final recovery of histostructure and antioxidative indices indicated that ovarian efficient antioxidant defense system might be an important mechanism of zebrafish to counteract MC-LR. Although the negative effects of MC-LR can be overcome by ovarian antioxidant system in this study, the potential reproductive risks of MC-LR should not be neglected because of its wide occurrence. (C) 2014 Elsevier B.V. All rights reserved.