Defective T-cell surface antigen expression after mitogen stimulation. An index of lymphocyte dysfunction after controlled murine injury.
Defective T-cell surface antigen expression after mitogen stimulation. An index of lymphocyte dysfunction after controlled murine injury.
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有丝分裂原刺激后 T 细胞表面抗原表达缺陷。
DOI：
10.1097/00000658-198901000-00016
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发表时间：
1989
影响因子：
9
通讯作者：
Ozkan,N
中科院分区：
文献类型：
--
作者：
Gadd,MA;Hansbrough,JF;Hoyt,DB;Ozkan,N
通过使用单克隆抗体和双色流式细胞术测量细胞表面标记物的时间表达，研究了 25% 体表面积烧伤或后肢截肢后凝集素刺激的培养物中小鼠脾 T 细胞的活化。淋巴细胞活化已被证明伴随着新表面抗原的出现，包括白介素 2 (IL-2) 受体 (IL-2R) 和 Ia，以及共表达辅助 (Th) 和抑制 (Ts) 表面标记的细胞的出现。 IL-2R 已被证明在 DNA 合成之前早期出现在受刺激的细胞上，而 Ia 出现较晚。在丝裂原刺激培养的第 0 小时以及 24、48 和 72 小时后分析表面标记（L3T4、Lyt2、Ia 和 IL-2R）。烧伤后Th (L3T4+) 和Ts (Lyt-2+) 群体中IL-2R 和Ia 的出现显着降低，但肌肉骨骼损伤后变化很小。此外，烧伤衍生细胞中 L3T4/Lyt2 抗原的共表达显着减少。烧伤动物的血清通过刺激正常细胞引起表面抗原表达抑制。当将重组 IL-2 添加到烧伤衍生细胞培养物中时，在培养过程中不会增加这些表面标记的表达，也不会改善增殖。
Murine spleen T-cell activation in lectin-stimulated cultures after 25% body surface area burn injury or hind-limb amputation was studied by measuring the temporal expression of cell surface markers using monoclonal antibodies and two-color flow cytometry. Lymphocyte activation has been shown to be accompanied by the appearance of new surface antigens, including Interleukin-2 (IL-2) deceptor (IL-2R) and Ia, and emergence of cells that coexpress helper (Th) and suppressor (Ts) surface markers. IL-2R has been shown to appear early on stimulated cells, before DNA synthesis, whereas Ia appears later. Surface markers (L3T4, Lyt2, Ia, and IL-2R) were analyzed at time 0 and after 24, 48, and 72 hours of mitogen-stimulated culture. The appearance of IL-2R and Ia on Th (L3T4+) and Ts (Lyt-2+) populations was markedly depressed after burn injury, but minimal changes were seen after musculoskeletal injury. In addition, coexpression of L3T4/Lyt2 antigens was markedly reduced in burn-derived cells. Serum from burn-injured animals caused depression of surface antigen expression by stimulated normal cells. Recombinant IL-2, when added to burn-derived cell cultures, did not increase expression of these surface markers during culture, nor did it improve proliferation.