Polysaccharide production by cultured B-16 mouse melanoma cells.
Polysaccharide production by cultured B-16 mouse melanoma cells.
复制标题
培养的 B-16 小鼠黑色素瘤细胞产生多糖。
DOI：
10.1021/bi00703a028
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发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
Eugene A. Davidson
中科院分区：
文献类型：
--
作者：
C. Satoh;John Banks;Pamela Horst;John W. Kreider;Eugene A. Davidson
材料和方法用于这些实验的B-16黑色素瘤细胞系的起源先前已有描述（Kreider等，1973年）。从缅因州巴尔港的杰克逊实验室保存的储备B-16肿瘤中分离无黑素克隆。克隆分离株通过玻璃瓶获得，来自米尔顿大学生物化学和病理学系。宾夕法尼亚州州立大学好时医学中心，好时，宾夕法尼亚州17033。1973年9月4日收到。这项工作得到了公共卫生服务赠款CA-15483和CA-11097以及公共卫生服务合同70-2024的部分支持。酸，一种占虹膜黑素细胞总标记部分约一半的产物。不同硫酸盐含量的高分子量软骨素，主要是4-硫酸盐，是克隆系的主要产物;在虹膜培养物中未检测到这种多糖。硫酸肝素存在于所有细胞中，但在致瘤性克隆中含量略高。芯片方法（Martin，1973）;所得的线仅产生非常少量的黑色素。所有的细胞都常规地在16盎司的处方瓶中繁殖，并用含有厄尔盐的最小量必需培养基、补充有非必需氨基酸的10%热灭活胎牛血清、丙酮酸钠和两倍于通常浓度的维生素。
Materials and MethodsThe origins of the B-16 melanotic melanoma cell line used in these experiments have been previously described (Kreider et al., 1973). The amelanotic clone was isolated from the stock B-16 tumor maintained by the Jackson Laboratory, Bar Harbor, Maine. Clonal isolates were obtained by the glass t From the Departments of Biological Chemistry and Pathology, The Milton S. Hershey Medical Center of ThePennsylvania State University, Hershey, Pennsylvania 17033. Received September 4, 1973. This work was supported in part by Public Health Service Grants CA-15483 and CA-11097 and by Public Health Service Contract 70-2024. acid, a product which accounts for about half of the total labeled fraction for the iris melanocytes. A high molecular weight chondroitin of varying sulfate content, predominantly 4-sulfate, is a major product of the cloned lines; this poly-saccharide was not detected in the iris cultures. Heparitin sulfate was present in all cells but appearedto be somewhat higher in content for the tumorigenic clones. chip method (Martin, 1973); the resulting line produces only very small amounts of melanin. All cells were routinely propagated in 16-oz prescription bottles and fed with mini-mum essential mediumwith Earle's salts, 10% heat-inactivated fetal calf serum supplemented with nonessential amino acids, sodium pyruvate, and twice the usual concentration of vita-mins.